Actin filaments modulate both stomatal opening and inward K+-channel activities in guard cells of Vicia faba L.

Jae Ung Hwang, Sujeoung Suh, Hanju Yi, Jimok Kim, Youngsook Lee

Research output: Contribution to journalArticle

112 Citations (Scopus)

Abstract

Actin antagonists have previously been shown to alter responses of Commelina communis stomata to physiological stimuli, implicating actin filaments in the control of guard cell volume changes (M. Kim, P.K. Hepler, S.-O. Eun, K.S. Ha, Y. Lee [1995] Plant Physiol 109: 1077-1084). Since K+ channels in the guard cell play an important role in stomatal movements, we examined the possible regulation of K+-channel activities by the state of actin polymerization. Agents affecting actin polymerization altered light-induced stomatal opening and inward K+-channel activities measured by patch clamping in Vicia faba. Cytochalasin D, which induces depolymerization of actin filaments, promoted light-induced stomatal opening and potentiated the inward K+ current in guard cell protoplasts. Phalloidin, a stabilizer of filamentous actin, inhibited both light-induced stomatal opening and inward K+ current. Inward K+-channel activities in outside-out membrane patches showed responses to these agents that support results at the whole-cell current level, suggesting that cytochalasin D facilitates and phalloidin inhibits K+ influx in intact guard cells, thus resulting in enhancement and inhibition of stomatal opening, respectively. To our knowledge, this is the first report that provides evidence that actin filaments may regulate an important physiological process by modulating the activities of ion channels in plant cells.

Original languageEnglish (US)
Pages (from-to)335-342
Number of pages8
JournalPlant Physiology
Volume115
Issue number2
DOIs
StatePublished - Jan 1 1997

Fingerprint

Vicia faba
potassium channels
guard cells
microfilaments
Actin Cytoskeleton
actin
Actins
cytochalasin D
Phalloidine
Cytochalasin D
Light
Polymerization
polymerization
Commelina
Commelina communis
Physiological Phenomena
stomatal movement
Protoplasts
depolymerization
Plant Cells

ASJC Scopus subject areas

  • Physiology
  • Genetics
  • Plant Science

Cite this

Actin filaments modulate both stomatal opening and inward K+-channel activities in guard cells of Vicia faba L. / Hwang, Jae Ung; Suh, Sujeoung; Yi, Hanju; Kim, Jimok; Lee, Youngsook.

In: Plant Physiology, Vol. 115, No. 2, 01.01.1997, p. 335-342.

Research output: Contribution to journalArticle

Hwang, Jae Ung ; Suh, Sujeoung ; Yi, Hanju ; Kim, Jimok ; Lee, Youngsook. / Actin filaments modulate both stomatal opening and inward K+-channel activities in guard cells of Vicia faba L. In: Plant Physiology. 1997 ; Vol. 115, No. 2. pp. 335-342.
@article{9d8365301a1747999c5ed0cafe1fa5ee,
title = "Actin filaments modulate both stomatal opening and inward K+-channel activities in guard cells of Vicia faba L.",
abstract = "Actin antagonists have previously been shown to alter responses of Commelina communis stomata to physiological stimuli, implicating actin filaments in the control of guard cell volume changes (M. Kim, P.K. Hepler, S.-O. Eun, K.S. Ha, Y. Lee [1995] Plant Physiol 109: 1077-1084). Since K+ channels in the guard cell play an important role in stomatal movements, we examined the possible regulation of K+-channel activities by the state of actin polymerization. Agents affecting actin polymerization altered light-induced stomatal opening and inward K+-channel activities measured by patch clamping in Vicia faba. Cytochalasin D, which induces depolymerization of actin filaments, promoted light-induced stomatal opening and potentiated the inward K+ current in guard cell protoplasts. Phalloidin, a stabilizer of filamentous actin, inhibited both light-induced stomatal opening and inward K+ current. Inward K+-channel activities in outside-out membrane patches showed responses to these agents that support results at the whole-cell current level, suggesting that cytochalasin D facilitates and phalloidin inhibits K+ influx in intact guard cells, thus resulting in enhancement and inhibition of stomatal opening, respectively. To our knowledge, this is the first report that provides evidence that actin filaments may regulate an important physiological process by modulating the activities of ion channels in plant cells.",
author = "Hwang, {Jae Ung} and Sujeoung Suh and Hanju Yi and Jimok Kim and Youngsook Lee",
year = "1997",
month = "1",
day = "1",
doi = "10.1104/pp.115.2.335",
language = "English (US)",
volume = "115",
pages = "335--342",
journal = "Plant Physiology",
issn = "0032-0889",
publisher = "American Society of Plant Biologists",
number = "2",

}

TY - JOUR

T1 - Actin filaments modulate both stomatal opening and inward K+-channel activities in guard cells of Vicia faba L.

AU - Hwang, Jae Ung

AU - Suh, Sujeoung

AU - Yi, Hanju

AU - Kim, Jimok

AU - Lee, Youngsook

PY - 1997/1/1

Y1 - 1997/1/1

N2 - Actin antagonists have previously been shown to alter responses of Commelina communis stomata to physiological stimuli, implicating actin filaments in the control of guard cell volume changes (M. Kim, P.K. Hepler, S.-O. Eun, K.S. Ha, Y. Lee [1995] Plant Physiol 109: 1077-1084). Since K+ channels in the guard cell play an important role in stomatal movements, we examined the possible regulation of K+-channel activities by the state of actin polymerization. Agents affecting actin polymerization altered light-induced stomatal opening and inward K+-channel activities measured by patch clamping in Vicia faba. Cytochalasin D, which induces depolymerization of actin filaments, promoted light-induced stomatal opening and potentiated the inward K+ current in guard cell protoplasts. Phalloidin, a stabilizer of filamentous actin, inhibited both light-induced stomatal opening and inward K+ current. Inward K+-channel activities in outside-out membrane patches showed responses to these agents that support results at the whole-cell current level, suggesting that cytochalasin D facilitates and phalloidin inhibits K+ influx in intact guard cells, thus resulting in enhancement and inhibition of stomatal opening, respectively. To our knowledge, this is the first report that provides evidence that actin filaments may regulate an important physiological process by modulating the activities of ion channels in plant cells.

AB - Actin antagonists have previously been shown to alter responses of Commelina communis stomata to physiological stimuli, implicating actin filaments in the control of guard cell volume changes (M. Kim, P.K. Hepler, S.-O. Eun, K.S. Ha, Y. Lee [1995] Plant Physiol 109: 1077-1084). Since K+ channels in the guard cell play an important role in stomatal movements, we examined the possible regulation of K+-channel activities by the state of actin polymerization. Agents affecting actin polymerization altered light-induced stomatal opening and inward K+-channel activities measured by patch clamping in Vicia faba. Cytochalasin D, which induces depolymerization of actin filaments, promoted light-induced stomatal opening and potentiated the inward K+ current in guard cell protoplasts. Phalloidin, a stabilizer of filamentous actin, inhibited both light-induced stomatal opening and inward K+ current. Inward K+-channel activities in outside-out membrane patches showed responses to these agents that support results at the whole-cell current level, suggesting that cytochalasin D facilitates and phalloidin inhibits K+ influx in intact guard cells, thus resulting in enhancement and inhibition of stomatal opening, respectively. To our knowledge, this is the first report that provides evidence that actin filaments may regulate an important physiological process by modulating the activities of ion channels in plant cells.

UR - http://www.scopus.com/inward/record.url?scp=0031401176&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031401176&partnerID=8YFLogxK

U2 - 10.1104/pp.115.2.335

DO - 10.1104/pp.115.2.335

M3 - Article

VL - 115

SP - 335

EP - 342

JO - Plant Physiology

JF - Plant Physiology

SN - 0032-0889

IS - 2

ER -