ACTION SPECTRA FOR KILLING NON‐DIVIDING NORMAL HUMAN AND XERODERMA PIGMENTOSUM CELLS

G. J. Kantor, John C. Sutherland, R. B. Setlow

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

Abstract— Non‐dividing human cells degenerate and eventually detach from a culture vessel surface when exposed to UV light. Action spectra for this kind of cell inactivation were determined using eight monochromatic wavelengths from 240 to 313 nm and both a normal DNA excision‐repair‐proficient strain and a repair‐deficient Xeroderma pigmentosum (XP12BE) strain. The action spectra for both strains have similar shapes with a broad peak between 254 and 280 nm followed by a steep decline at wavelengths greater than 280 nm. The relative action spectra are similar to those for inactivation of reproductive capacity and pyrimidine dimer formation in rodent cells suggesting that the critical target and critical damage for inactivation of non‐dividing human cells is DNA and damage to DNA, respectively. Normal repair‐proficient cells are 5–7 times more resistant at all wavelengths, based on a comparison of Do values, than repair‐deficient XP12BE cells, supporting the conclusion that the inactivating damage at all wavelengths is to DNA.

Original languageEnglish (US)
Pages (from-to)459-464
Number of pages6
JournalPhotochemistry and Photobiology
Volume31
Issue number5
DOIs
StatePublished - Jan 1 1980
Externally publishedYes

Fingerprint

Xeroderma Pigmentosum
deoxyribonucleic acid
deactivation
Wavelength
DNA
damage
cells
wavelengths
Cells
Pyrimidine Dimers
rodents
pyrimidines
Cell culture
Ultraviolet radiation
vessels
dimers
Ultraviolet Rays
DNA Damage
Action Spectrum
Rodentia

ASJC Scopus subject areas

  • Medicine(all)
  • Biochemistry
  • Physical and Theoretical Chemistry

Cite this

ACTION SPECTRA FOR KILLING NON‐DIVIDING NORMAL HUMAN AND XERODERMA PIGMENTOSUM CELLS. / Kantor, G. J.; Sutherland, John C.; Setlow, R. B.

In: Photochemistry and Photobiology, Vol. 31, No. 5, 01.01.1980, p. 459-464.

Research output: Contribution to journalArticle

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AB - Abstract— Non‐dividing human cells degenerate and eventually detach from a culture vessel surface when exposed to UV light. Action spectra for this kind of cell inactivation were determined using eight monochromatic wavelengths from 240 to 313 nm and both a normal DNA excision‐repair‐proficient strain and a repair‐deficient Xeroderma pigmentosum (XP12BE) strain. The action spectra for both strains have similar shapes with a broad peak between 254 and 280 nm followed by a steep decline at wavelengths greater than 280 nm. The relative action spectra are similar to those for inactivation of reproductive capacity and pyrimidine dimer formation in rodent cells suggesting that the critical target and critical damage for inactivation of non‐dividing human cells is DNA and damage to DNA, respectively. Normal repair‐proficient cells are 5–7 times more resistant at all wavelengths, based on a comparison of Do values, than repair‐deficient XP12BE cells, supporting the conclusion that the inactivating damage at all wavelengths is to DNA.

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