Action spectra for ultraviolet photosensitized killing of mammalian cells by misonidazole and para-nitroacetophenone

G. J. Fisher, B. M. Sutherland, J. C. Sutherland

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9 Scopus citations

Abstract

V-79 Chinese hamster fibroblasts in monolayer culture were exposed to ultraviolet radiation at 313, 334, 365, 380 and 405 nm in the presence of either misonidazole or para-nitroacetophenone, drugs which act as both photosensitizers and radiosensitizers of cell killing. Survival was measured by a colony-forming assay. The resulting action spectra for cell death photosensitized by the drugs (the reciprocals of the exposures required at each wavelength to reduce cell survival to a given level) closely match their absorption spectra over a range of three orders of magnitude. These results demonstrate that cells can be killed upon excitation of misonidazole or para-nitroacetophenone in the absence of any other types of energy deposition or biomolecular damage.

Original languageEnglish (US)
Pages (from-to)231-238
Number of pages8
JournalRadiation research
Volume94
Issue number2
DOIs
Publication statusPublished - Jan 1 1983
Externally publishedYes

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ASJC Scopus subject areas

  • Biophysics
  • Radiation
  • Radiology Nuclear Medicine and imaging

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