Action spectra for ultraviolet photosensitized killing of mammalian cells by misonidazole and para-nitroacetophenone

G. J. Fisher, B. M. Sutherland, John C. Sutherland

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

V-79 Chinese hamster fibroblasts in monolayer culture were exposed to ultraviolet radiation at 313, 334, 365, 380 and 405 nm in the presence of either misonidazole or para-nitroacetophenone, drugs which act as both photosensitizers and radiosensitizers of cell killing. Survival was measured by a colony-forming assay. The resulting action spectra for cell death photosensitized by the drugs (the reciprocals of the exposures required at each wavelength to reduce cell survival to a given level) closely match their absorption spectra over a range of three orders of magnitude. These results demonstrate that cells can be killed upon excitation of misonidazole or para-nitroacetophenone in the absence of any other types of energy deposition or biomolecular damage.

Original languageEnglish (US)
Pages (from-to)231-238
Number of pages8
JournalRadiation Research
Volume94
Issue number2
DOIs
StatePublished - Jan 1 1983
Externally publishedYes

Fingerprint

Misonidazole
Photosensitizing Agents
drugs
Cricetulus
Pharmaceutical Preparations
Cell Survival
Cell Death
hamsters
Fibroblasts
fibroblasts
Radiation
death
ultraviolet radiation
damage
absorption spectra
cells
wavelengths
excitation
Action Spectrum
energy

ASJC Scopus subject areas

  • Biophysics
  • Radiation
  • Radiology Nuclear Medicine and imaging

Cite this

Action spectra for ultraviolet photosensitized killing of mammalian cells by misonidazole and para-nitroacetophenone. / Fisher, G. J.; Sutherland, B. M.; Sutherland, John C.

In: Radiation Research, Vol. 94, No. 2, 01.01.1983, p. 231-238.

Research output: Contribution to journalArticle

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