Activated charcoal filter effectively reduces p-benzosemiquinone from the mainstream cigarette smoke and prevents emphysema

Neekkan Dey, Archita Das, Arunava Ghosh, Indu B. Chatterjee

Research output: Contribution to journalArticle

Abstract

In this paper, we have made a comparative evaluation of the cytotoxicity and pathophysiological effects of mainstream smoke from cellulose acetate (CA)-filtered cigarettes with that of charcoal-filtered cigarettes developed in our laboratory. Previously, we had demonstrated that the mainstream smoke from an Indian CA-filtered commercial cigarette contains p-benzosemiquinone (p-BSQ), a major, highly toxic, long-lived water-soluble radical. Here, we have examined 16 brands of different CA-filtered cigarettes including Kentucky research cigarettes, and observed that mainstream smoke from all the cigarettes contains substantial amounts of p-BSQ (100-200 μg/cigarette). We also show that when the CA filter is replaced by a charcoal filter, the amount of p-BSQ in the mainstream smoke is reduced by 73-80%, which is accompanied by a reduction of carbonyl formation in bovine serum albumin to the extent of 70-90%. The charcoal filter also prevented cytotoxicity in A549 cells as evidenced by MTT assay, apoptosis as evidenced by FACS analysis, TUNEL assay, overexpression of Bax, activation of p53 and caspase 3, as well as emphysematous lung damage in a guinea pig model as seen by histology and morphometric analysis. The results indicate that the charcoal filter developed in our laboratory may protect smokers from cigarette smoke-induced cytotoxity, protein modification, apoptosis and emphysema.

Original languageEnglish (US)
Pages (from-to)217-230
Number of pages14
JournalJournal of Biosciences
Volume35
Issue number2
DOIs
StatePublished - Jun 1 2010
Externally publishedYes

Fingerprint

cigarettes
Charcoal
Emphysema
activated carbon
smoke
Smoke
Tobacco Products
cellulose acetate
charcoal
Cytotoxicity
cytotoxicity
Assays
apoptosis
Apoptosis
Histology
Poisons
In Situ Nick-End Labeling
assays
caspase-3
bovine serum albumin

Keywords

  • Apoptosis
  • Charcoal filter
  • Cigarette smoke
  • Emphysema
  • p-benzosemiquinone

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Activated charcoal filter effectively reduces p-benzosemiquinone from the mainstream cigarette smoke and prevents emphysema. / Dey, Neekkan; Das, Archita; Ghosh, Arunava; Chatterjee, Indu B.

In: Journal of Biosciences, Vol. 35, No. 2, 01.06.2010, p. 217-230.

Research output: Contribution to journalArticle

Dey, Neekkan ; Das, Archita ; Ghosh, Arunava ; Chatterjee, Indu B. / Activated charcoal filter effectively reduces p-benzosemiquinone from the mainstream cigarette smoke and prevents emphysema. In: Journal of Biosciences. 2010 ; Vol. 35, No. 2. pp. 217-230.
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