Activated notch causes deafness by promoting a supporting cell phenotype in developing auditory hair cells

Grace Savoy-Burke, Felicia A. Gilels, Wei Pan, Diana Pratt, Jianwen Que, Lin Gan, Patricia M. White, Amy E. Kiernan

Research output: Contribution to journalArticle

9 Scopus citations

Abstract

Purpose: To determine whether activated Notch can promote a supporting cell fate during sensory cell differentiation in the inner ear.

Methods: An activated form of the Notch1 receptor (NICD) was expressed in early differentiating hair cells using a Gfi1-Cre mouse allele. To determine the effects of activated Notch on developing hair cells, Gfi1-NICD animals and their littermate controls were assessed at 5 weeks for hearing by measuring auditory brainstem responses (ABRs) and distortion product otoacoustic emissions (DPOAEs). The differentiation of NICD-expressing hair cells was assessed at postnatal day (P) 6, 11 and 20, using histological and molecular markers for hair cells, as well as supporting cells/progenitor cells. We also examined whether the effects of Notch were mediated by SOX2, a gene expressed in supporting cells and a likely downstream target of Notch, by crossing an inducible form of SOX2 to the Gfi1-Cre.

Results: Activation of Notch1 in developing auditory hair cells causes profound deafness. The NICD-expressing hair cells switch off a number of hair cell markers and lose their characteristic morphology. Instead, NICD-expressing hair cells adopt a morphology resembling supporting cells and upregulate a number of supporting cell markers. These effects do not appear to be mediated by SOX2, because although expression of SOX2 caused some hearing impairment, the SOX2-expressing hair cells did not downregulate hair cell markers nor exhibit a supporting cell-like phenotype.

Conclusions: Our data show that Notch signaling inhibits hair cell differentiation and promotes a supporting cell-like phenotype, and that these effects are unlikely to be mediated by SOX2.

Original languageEnglish (US)
Article numbere108160
JournalPloS one
Volume9
Issue number9
DOIs
StatePublished - Sep 29 2014

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • General

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    Savoy-Burke, G., Gilels, F. A., Pan, W., Pratt, D., Que, J., Gan, L., White, P. M., & Kiernan, A. E. (2014). Activated notch causes deafness by promoting a supporting cell phenotype in developing auditory hair cells. PloS one, 9(9), [e108160]. https://doi.org/10.1371/journal.pone.0108160