Activated platelets contribute importantly to myocardial reperfusion injury

Yaqin Xu, Yuqing Huo, Marie Claire Toufektsian, Susan I. Ramos, Yongguang Ma, Ankit D. Tejani, Brent A. French, Zequan Yang

Research output: Contribution to journalArticle

90 Citations (Scopus)

Abstract

Platelets become activated during myocardial infarction (MI), but the direct contribution of activated platelets to myocardial reperfusion injury in vivo has yet to be reported. We tested the hypothesis that activated platelets contribute importantly to reperfusion injury during MI in mice. After 30 min of ischemia and 60 min of reperfusion, P-selectin knockout mice had a significantly smaller infarct size than that of wild-type mice P < 0.05). Platelets were detected by P-selectin antibody in the previously ischemic region of wild-type mice as early as 2 min postreperfusion after 45 min, but not 20 min, of ischemia. The appearance of neutrophils in the heart was delayed when compared with platelets. Flow cytometry showed that the number of activated platelets more than doubled after 45 min of ischemia when compared with 20 min of ischemia or sham treatment (P < 0.05). Platelet-rich or platelet-poor plasma was then transfused from either sham-operated or infarcted mice after 45 and 10 min of ischemia-reperfusion to mice undergoing 20 and 60 min of ischemia-reperfusion. Infarct size was increased by threefold and platelet accumulation was remarkably enhanced in mice treated with wild-type, MI-activated platelet-rich plasma but not in mice receiving either platelet-poor plasma from wild types or MI-activated platelet-rich plasma from P-selectin knockout mice. In conclusion, circulating platelets become activated early during reperfusion and their activation depends on the duration of the preceding coronary occlusion and is proportional to the extent of myocardial injury. Activated platelets play an important role in the process of myocardial ischemia-reperfusion injury, and platelet-derived P-selectin is a critical mediator.

Original languageEnglish (US)
Pages (from-to)H692-H699
JournalAmerican Journal of Physiology - Heart and Circulatory Physiology
Volume290
Issue number2
DOIs
StatePublished - Feb 1 2006

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Myocardial Reperfusion Injury
Blood Platelets
P-Selectin
Ischemia
Reperfusion
Myocardial Infarction
Platelet-Rich Plasma
Reperfusion Injury
Knockout Mice
Coronary Occlusion
Platelet Count
Myocardial Ischemia
Flow Cytometry
Neutrophils

Keywords

  • Knockout mice
  • P-selectin

ASJC Scopus subject areas

  • Physiology
  • Cardiology and Cardiovascular Medicine
  • Physiology (medical)

Cite this

Activated platelets contribute importantly to myocardial reperfusion injury. / Xu, Yaqin; Huo, Yuqing; Toufektsian, Marie Claire; Ramos, Susan I.; Ma, Yongguang; Tejani, Ankit D.; French, Brent A.; Yang, Zequan.

In: American Journal of Physiology - Heart and Circulatory Physiology, Vol. 290, No. 2, 01.02.2006, p. H692-H699.

Research output: Contribution to journalArticle

Xu, Yaqin ; Huo, Yuqing ; Toufektsian, Marie Claire ; Ramos, Susan I. ; Ma, Yongguang ; Tejani, Ankit D. ; French, Brent A. ; Yang, Zequan. / Activated platelets contribute importantly to myocardial reperfusion injury. In: American Journal of Physiology - Heart and Circulatory Physiology. 2006 ; Vol. 290, No. 2. pp. H692-H699.
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