Activation and autophosphorylation of apoptosis signal-regulating kinase 1 (ASK1) following cerebral ischemia in rat hippocampus

Quanguang Zhang, Guangyi Zhang

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

We investigated the expression, activation and autophosphorylation of apoptosis signal-regulating kinase 1 (ASK1) in rat hippocampus after cerebral ischemia. The in vitro kinase assay showed that ASK1 activity gradually increased while the autophosphorylation of ASK1 gradually reduced during 5, 15 and 30 min of cerebral ischemia. At various time points of reperfusion, the activation and autophosphorylation of ASK1 reached a high point at 30 min and reduced to basal level at 6 h and then slightly increased at 3 d compared with sham operation. Both of the increases of ASK1 activation and autophosphorylation were suppressed by N-acetylcysteine, a well-known antioxidant, which was administered to the Sprague-Dawley rat 20 min before cerebral ischemia. Immunoprecipitation and Western blotting assay showed that there was no obvious change in the amount of ASK1 at each time point compared with sham control. Our results suggest that ASK1 protein which is known as an upstream mediator of JNK/p38 mitogen-actived protein kinase (MAPK) activation may play an important role in signal transduction in response to ischemic stress, given the fact that activation of JNK/p38 MAPK and subsequent phosphorylation of c-Jun are involved in the apoptotic pathway in cerebral ischemia.

Original languageEnglish (US)
Pages (from-to)232-236
Number of pages5
JournalNeuroscience Letters
Volume329
Issue number2
DOIs
StatePublished - Aug 30 2002
Externally publishedYes

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MAP Kinase Kinase Kinase 5
Brain Ischemia
Hippocampus
Mitogens
Protein Kinases
Acetylcysteine
Immunoprecipitation
Reperfusion
Sprague Dawley Rats
Signal Transduction
Phosphotransferases
Antioxidants
Western Blotting
Phosphorylation

Keywords

  • Activation
  • Apoptosis signal-regulating kinase 1 (ASK1)
  • Autophosphorylation
  • Cerebral ischemia
  • Expression
  • Hippocampus
  • N-acetylcysteine
  • Rat

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Activation and autophosphorylation of apoptosis signal-regulating kinase 1 (ASK1) following cerebral ischemia in rat hippocampus. / Zhang, Quanguang; Zhang, Guangyi.

In: Neuroscience Letters, Vol. 329, No. 2, 30.08.2002, p. 232-236.

Research output: Contribution to journalArticle

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