Activation and Loading of the Starter Unit during Thiocoraline Biosynthesis

Shogo Mori, Sanjib K. Shrestha, Javier Fernández, María Álvarez San Millán, Atefeh Garzan, Ahmad H. Al-Mestarihi, Felipe Lombó, Sylvie Garneau-Tsodikova

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

The initiation of the nonribosomal peptide synthetase (NRPS) assembly of the bisintercalator natural product thiocoraline involves key enzymatic steps for AMP activation and carrier protein loading of the starter unit 3-hydroxyquinaldic acid (3HQA). Gene cluster data combined with protein sequence homology analysis originally led us to propose that TioJ could be responsible for the AMP activation step, whereas TioO could act as the thiolation (T) domain, facilitating the transfer of 3HQA to the next NRPS module, TioR. Herein, we confirmed the involvement of TioJ in thiocoraline biosynthesis by tioJ knockout and in vitro activation of 3HQA studies. However, we demonstrated that TioJ-activated 3HQA is not loaded onto the T domain TioO, as originally believed, but instead onto a fatty acid synthase (FAS) acyl carrier protein (ACP) domain FabC, which is located outside of the thiocoraline gene cluster. We showed a strong interaction between TioJ and FabC. By generating TioJ point mutants mimicking the active site of highly homologous enzymes activating different molecules, we showed that the identity of the substrate activated by adenylation domains such as TioJ is not determined by only the active site residues that directly interact with the substrate. The insights gained from these enzymatic transformations are valuable in the efforts toward deciphering the complete biosynthetic pathway of thiocoraline and bisintercalators in general.

Original languageEnglish (US)
Pages (from-to)4457-4467
Number of pages11
JournalBiochemistry
Volume56
Issue number34
DOIs
StatePublished - Aug 29 2017
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry

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