Activation of gelatinolytic/collagenolytic activity in dentin by self-etching adhesives

Yoshihiro Nishitani, Masahiro Yoshiyama, Bakul Wadgaonkar, Lorenzo Breschi, Ferdinando Mannello, Annalisa Mazzoni, Ricardo M. Carvalho, Leo Tjäderhane, Franklin Chi Meng Tay, David Henry Pashley

Research output: Contribution to journalArticle

243 Citations (Scopus)

Abstract

Mild acids are known to activate dentin matrix metalloproteinase (MMPs). All self-etching dental adhesives are acidic (pH 1.5-2.7) and may activate dentin MMPs. The purpose of this study was to compare the ability of several all-in-one adhesives to activate gelatinolytic and collagenolytic activities in powdered mineralized dentin. Powdered dentin made from human teeth was mixed with all-in-one adhesives (Clearfil Tri-S Bond, G-Bond, Adper Prompt L-Pop) or a self-etching primer (Clearfil SE Bond primer) for varying times and then the reaction was stopped by extracting the adhesives using acetone. Fresh untreated mineralized dentin powder had a gelatinolytic activity of 3.31 ± 0.39 relative fluorescent units (RFU) per mg dry weight (24 h) that increased, over storage time, to 87.5 RFU mg-1 (24 h) after 6-8 wk. When fresh powder was treated with acidic Tri-S Bond, the gelatinolytic activity increased from 3.24 ± 0.70 RFU mg-1 to > 112.5 RFU mg-1 (24 h) after 20 min and then remained unchanged. Monomers with lower pH values produced less activity. There was a significant, direct correlation between gelatinolytic activity and pH, with Tri-S giving the highest activity. Coating dentin powder with Tri-S resin prevented fluorescent substrates from gaining access to the enzyme, even though it activated the enzyme. In conclusion, self-etch adhesives may activate latent MMP and increase the activity to near-maximum levels and contribute to the degradation of resin-dentin bonds over time.

Original languageEnglish (US)
Pages (from-to)160-166
Number of pages7
JournalEuropean Journal of Oral Sciences
Volume114
Issue number2
DOIs
StatePublished - Apr 1 2006

Fingerprint

Dentin
Adhesives
Matrix Metalloproteinases
Powders
Dental Cements
Enzymes
Acetone
Tooth
Weights and Measures
Acids

Keywords

  • Acrylics
  • Collagen
  • Dentin
  • Matrix metalloproteinase

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

Nishitani, Y., Yoshiyama, M., Wadgaonkar, B., Breschi, L., Mannello, F., Mazzoni, A., ... Pashley, D. H. (2006). Activation of gelatinolytic/collagenolytic activity in dentin by self-etching adhesives. European Journal of Oral Sciences, 114(2), 160-166. https://doi.org/10.1111/j.1600-0722.2006.00342.x

Activation of gelatinolytic/collagenolytic activity in dentin by self-etching adhesives. / Nishitani, Yoshihiro; Yoshiyama, Masahiro; Wadgaonkar, Bakul; Breschi, Lorenzo; Mannello, Ferdinando; Mazzoni, Annalisa; Carvalho, Ricardo M.; Tjäderhane, Leo; Tay, Franklin Chi Meng; Pashley, David Henry.

In: European Journal of Oral Sciences, Vol. 114, No. 2, 01.04.2006, p. 160-166.

Research output: Contribution to journalArticle

Nishitani, Y, Yoshiyama, M, Wadgaonkar, B, Breschi, L, Mannello, F, Mazzoni, A, Carvalho, RM, Tjäderhane, L, Tay, FCM & Pashley, DH 2006, 'Activation of gelatinolytic/collagenolytic activity in dentin by self-etching adhesives', European Journal of Oral Sciences, vol. 114, no. 2, pp. 160-166. https://doi.org/10.1111/j.1600-0722.2006.00342.x
Nishitani, Yoshihiro ; Yoshiyama, Masahiro ; Wadgaonkar, Bakul ; Breschi, Lorenzo ; Mannello, Ferdinando ; Mazzoni, Annalisa ; Carvalho, Ricardo M. ; Tjäderhane, Leo ; Tay, Franklin Chi Meng ; Pashley, David Henry. / Activation of gelatinolytic/collagenolytic activity in dentin by self-etching adhesives. In: European Journal of Oral Sciences. 2006 ; Vol. 114, No. 2. pp. 160-166.
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abstract = "Mild acids are known to activate dentin matrix metalloproteinase (MMPs). All self-etching dental adhesives are acidic (pH 1.5-2.7) and may activate dentin MMPs. The purpose of this study was to compare the ability of several all-in-one adhesives to activate gelatinolytic and collagenolytic activities in powdered mineralized dentin. Powdered dentin made from human teeth was mixed with all-in-one adhesives (Clearfil Tri-S Bond, G-Bond, Adper Prompt L-Pop) or a self-etching primer (Clearfil SE Bond primer) for varying times and then the reaction was stopped by extracting the adhesives using acetone. Fresh untreated mineralized dentin powder had a gelatinolytic activity of 3.31 ± 0.39 relative fluorescent units (RFU) per mg dry weight (24 h) that increased, over storage time, to 87.5 RFU mg-1 (24 h) after 6-8 wk. When fresh powder was treated with acidic Tri-S Bond, the gelatinolytic activity increased from 3.24 ± 0.70 RFU mg-1 to > 112.5 RFU mg-1 (24 h) after 20 min and then remained unchanged. Monomers with lower pH values produced less activity. There was a significant, direct correlation between gelatinolytic activity and pH, with Tri-S giving the highest activity. Coating dentin powder with Tri-S resin prevented fluorescent substrates from gaining access to the enzyme, even though it activated the enzyme. In conclusion, self-etch adhesives may activate latent MMP and increase the activity to near-maximum levels and contribute to the degradation of resin-dentin bonds over time.",
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