Activation of postsynaptic GABAB receptors modulate the firing activity of supraoptic oxytocin and vasopressin neurones

Role of calcium channels

Y. Li, Javier E. Stern

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Oxytocin and vasopressin release from neurohypophysial terminals is closely related to the firing activity of magnocellular neurones in the supraoptic (SON) and paraventricular nuclei. It is well established that activation of GABAA receptors potently inhibits the activity of SON neurones and, thus, hormone release. However, whether postsynaptic GABAB receptors are expressed in magnocellular neurones, and the role they play in controlling their firing activity, is still controversial. In the present work, we combined immunohistochemical and electrophysiological techniques to determine whether activation of GABAB receptors in identified oxytocin and vasopressin neurones modulates their firing activity. Patch-clamp recordings from SON neurones were obtained either in the slice preparation or from acutely dissociated neurones. Activation of GABAB receptors with the selective agonist baclofen (10 μM inhibited voltage-gated Ca2+ currents, reduced the duration of individual action potentials, as well as the magnitude of the hyperpolarizing after-potential. SON firing activity was reduced by baclofen, and effect that was accompanied by a small membrane hyperpolarization. The inhibition of firing discharge persisted in the presence of synaptic blockade media, and was also observed in acutely dissociated SON neurones. Finally, GABAB-mediated modulation of firing activity was largely blocked by the Ca2+ channel blocker Co2+ (2 mm). In general, baclofen modulatory actions were significantly larger, or observed more predominantly, in vasopressin neurones. In summary, these results support the expression of functional postsynaptic GABAB receptors in SON neurones, activation of which efficiently modulates neuronal excitability, in a Ca2+- and cell-type dependent manner.

Original languageEnglish (US)
Pages (from-to)119-130
Number of pages12
JournalJournal of Neuroendocrinology
Volume16
Issue number2
DOIs
StatePublished - Feb 1 2004

Fingerprint

Oxytocin
Calcium Channels
Vasopressins
Neurons
Baclofen
Supraoptic Nucleus
Paraventricular Hypothalamic Nucleus
GABA-A Receptors
Action Potentials
Hormones
Membranes

Keywords

  • GABA
  • Hypothalamus
  • Magnocellular
  • Neuroendocrine
  • Synaptic

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Endocrinology
  • Endocrine and Autonomic Systems
  • Cellular and Molecular Neuroscience

Cite this

Activation of postsynaptic GABAB receptors modulate the firing activity of supraoptic oxytocin and vasopressin neurones : Role of calcium channels. / Li, Y.; Stern, Javier E.

In: Journal of Neuroendocrinology, Vol. 16, No. 2, 01.02.2004, p. 119-130.

Research output: Contribution to journalArticle

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