Abstract
During Drosophila embryogenesis, establishment of ventral and lateral cell fates requires spatial regulation of an extracellular serine protease cascade composed of Nudel, Gastrulation Defective (GD), Snake, and Easter. Pipe, a sulfotransferase expressed ventrally during oogenesis, sulfates secreted targets that somehow confer positive spatial input to this cascade. Nudel and GD activation are pipe-independent, while Easter activation requires pipe. The effect of pipe on Snake activation has been unknown. Here we show that Snake activation is cascade-dependent but pipe-independent. These findings support a conclusion that Snake's activation of Easter is the first spatially regulated step in the dorsoventral protease cascade.
Original language | English (US) |
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Pages (from-to) | 3557-3560 |
Number of pages | 4 |
Journal | FEBS Letters |
Volume | 584 |
Issue number | 16 |
DOIs | |
State | Published - Aug 2010 |
Keywords
- Dorsoventral polarity
- Drosophila
- Embryogenesis
- Fluorophosphonate (FP)-rhodamine
- Serine protease
- Snake
ASJC Scopus subject areas
- Biophysics
- Structural Biology
- Biochemistry
- Molecular Biology
- Genetics
- Cell Biology