Acute effects of thyroid hormones on the production of adrenal cAMP and corticosterone in male rats

Ming Jae Lo, Mei Mei Kau, Yen Hao Chen, Shiow Chwen Tsai, Yu Chung Chiao, Jiann Jong Chen, Charlie Liaw, Chien Chen Lu, Bu Pian Lee, Si Chih Chen, Victor S. Fang, Low Tone Ho, Paulus S. Wang

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Abstract

The acute effects of thyroid hormones on glucocorticoid secretion were studied. Venous blood samples were collected from male rats after they received intravenous 3,5,3'-triiodothyronine (T3) or thyroxine (T4). Zona fasciculata-reticularis (ZFR) cells were treated with adrenocorticotropic hormone (ACTH), T3, T4, ACTH plus T3, or ACTH plus T4 at 37°C for 2 h. Corticosterone concentrations in plasma and cell media, and also adenosine 3',5'-cyclic monophosphate (cAMP) production in ZFR cells in the presence of 3-isobutyl-1-methylxanthine, were determined. The effects of thyroid hormones on the activities of steroidogenic enzymes of ZFR cells were measured by the amounts of intermediate steroidal products separated by thin-layer chromatography. Administration of T3 and T4 suppressed the basal and the ACTH-stimulated levels of plasma corticosterone. In ZFR cells, both thyroid hormones inhibited ACTH-stimulated corticosterone secretion, but the basal corticosterone was inhibited only with T3 >10-10 M or T4 >10-8 M. Likewise, T3 or T4 at 10-7 M inhibited the basal- and ACTH-stimulated levels of intracellular cAMP. Physiological doses of T3 and T4 decreased the activities of 3β-hydroxysteroid dehydrogenase, 21-hydroxylase, and 11β- hydroxylase. These results suggest that thyroid hormones counteract ACTH in adrenal steroidogenesis through their inhibition of cAMP production in ZFR cells.

Original languageEnglish (US)
JournalAmerican Journal of Physiology
Volume274
Issue number2 PART 1
StatePublished - Feb 1 1998

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Zona Reticularis
Corticosterone
Zona Fasciculata
Thyroid Hormones
Adrenocorticotropic Hormone
Rats
3-Hydroxysteroid Dehydrogenases
Steroid 21-Hydroxylase
Plasmas
1-Methyl-3-isobutylxanthine
Thin layer chromatography
Triiodothyronine
Thin Layer Chromatography
Mixed Function Oxygenases
Plasma Cells
Thyroxine
Cyclic AMP
Glucocorticoids
Blood
Enzymes

Keywords

  • 3,5,3'-triiodothyronine
  • Adenosine 3',5'-cyclic monophosphate
  • P450c activity
  • Thyroxine
  • Zona fasciculata-reticularis cells

ASJC Scopus subject areas

  • Physiology
  • Endocrinology
  • Biochemistry

Cite this

Lo, M. J., Kau, M. M., Chen, Y. H., Tsai, S. C., Chiao, Y. C., Chen, J. J., ... Wang, P. S. (1998). Acute effects of thyroid hormones on the production of adrenal cAMP and corticosterone in male rats. American Journal of Physiology, 274(2 PART 1).

Acute effects of thyroid hormones on the production of adrenal cAMP and corticosterone in male rats. / Lo, Ming Jae; Kau, Mei Mei; Chen, Yen Hao; Tsai, Shiow Chwen; Chiao, Yu Chung; Chen, Jiann Jong; Liaw, Charlie; Lu, Chien Chen; Lee, Bu Pian; Chen, Si Chih; Fang, Victor S.; Ho, Low Tone; Wang, Paulus S.

In: American Journal of Physiology, Vol. 274, No. 2 PART 1, 01.02.1998.

Research output: Contribution to journalArticle

Lo, MJ, Kau, MM, Chen, YH, Tsai, SC, Chiao, YC, Chen, JJ, Liaw, C, Lu, CC, Lee, BP, Chen, SC, Fang, VS, Ho, LT & Wang, PS 1998, 'Acute effects of thyroid hormones on the production of adrenal cAMP and corticosterone in male rats', American Journal of Physiology, vol. 274, no. 2 PART 1.
Lo MJ, Kau MM, Chen YH, Tsai SC, Chiao YC, Chen JJ et al. Acute effects of thyroid hormones on the production of adrenal cAMP and corticosterone in male rats. American Journal of Physiology. 1998 Feb 1;274(2 PART 1).
Lo, Ming Jae ; Kau, Mei Mei ; Chen, Yen Hao ; Tsai, Shiow Chwen ; Chiao, Yu Chung ; Chen, Jiann Jong ; Liaw, Charlie ; Lu, Chien Chen ; Lee, Bu Pian ; Chen, Si Chih ; Fang, Victor S. ; Ho, Low Tone ; Wang, Paulus S. / Acute effects of thyroid hormones on the production of adrenal cAMP and corticosterone in male rats. In: American Journal of Physiology. 1998 ; Vol. 274, No. 2 PART 1.
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abstract = "The acute effects of thyroid hormones on glucocorticoid secretion were studied. Venous blood samples were collected from male rats after they received intravenous 3,5,3'-triiodothyronine (T3) or thyroxine (T4). Zona fasciculata-reticularis (ZFR) cells were treated with adrenocorticotropic hormone (ACTH), T3, T4, ACTH plus T3, or ACTH plus T4 at 37°C for 2 h. Corticosterone concentrations in plasma and cell media, and also adenosine 3',5'-cyclic monophosphate (cAMP) production in ZFR cells in the presence of 3-isobutyl-1-methylxanthine, were determined. The effects of thyroid hormones on the activities of steroidogenic enzymes of ZFR cells were measured by the amounts of intermediate steroidal products separated by thin-layer chromatography. Administration of T3 and T4 suppressed the basal and the ACTH-stimulated levels of plasma corticosterone. In ZFR cells, both thyroid hormones inhibited ACTH-stimulated corticosterone secretion, but the basal corticosterone was inhibited only with T3 >10-10 M or T4 >10-8 M. Likewise, T3 or T4 at 10-7 M inhibited the basal- and ACTH-stimulated levels of intracellular cAMP. Physiological doses of T3 and T4 decreased the activities of 3β-hydroxysteroid dehydrogenase, 21-hydroxylase, and 11β- hydroxylase. These results suggest that thyroid hormones counteract ACTH in adrenal steroidogenesis through their inhibition of cAMP production in ZFR cells.",
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