Acute pressor response to psychosocial stress is dependent on endothelium-derived endothelin-1

Brandon M. Fox, Bryan K. Becker, Analia S. Loria, Kelly A. Hyndman, Chunhua Jin, Hannah Clark, Robin F Johns, Masashi Yanagisawa, David M. Pollock, Jennifer S. Pollock

Research output: Contribution to journalArticle

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Abstract

Background--Acute psychosocial stress provokes increases in circulating endothelin-1 (ET-1) levels in humans and animal models. However, key questions about the physiological function and cellular source of stress-induced ET-1 remain unanswered. We hypothesized that endothelium-derived ET-1 contributes to the acute pressor response to stress via activation of the endothelin A receptor. Methods and Results--Adult male vascular endothelium-specific ET-1 knockout mice and control mice that were homozygous for the floxed allele were exposed to acute psychosocial stress in the form of cage switch stress (CSS), with blood pressure measured by telemetry. An acute pressor response was elicited by CSS in both genotypes; however, this response was significantly blunted in vascular endothelium-specific ET-1 knockout mice compared with control mice that were homozygous for the floxed allele. In mice pretreated for 3 days with the endothelin A antagonist, ABT-627, or the dual endothelin A/B receptor antagonist, A-182086, the pressor response to CSS was similar between genotypes. CSS significantly increased plasma ET-1 levels in control mice that were homozygous for the floxed allele. CSS failed to elicit an increase in plasma ET-1 in vascular endothelium-specific ET-1 knockout mice. Telemetry frequency domain analyses suggested similar autonomic responses to stress between genotypes, and isolated resistance arteries demonstrated similar sensitivity to a1-adrenergic receptor-mediated vasoconstriction. Conclusions--These findings specify that acute stress-induced activation of endothelium-derived ET-1 and subsequent endothelin A receptor activation is a novel mediator of the blood pressure response to acute psychosocial stress.

LanguageEnglish (US)
Article numbere007863
JournalJournal of the American Heart Association
Volume7
Issue number4
DOIs
StatePublished - Feb 1 2018

Fingerprint

Endothelin-1
Endothelium
Vascular Endothelium
Knockout Mice
Endothelin A Receptors
Telemetry
Alleles
Genotype
Blood Pressure
Vasoconstriction
Adrenergic Receptors
Animal Models
Arteries

Keywords

  • Cage switch stress
  • Endothelin-1
  • Endothelium-derived factors
  • Psychosocial stress
  • Stress
  • Vascular endothelium-specific ET-1 knockout mice

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

Cite this

Fox, B. M., Becker, B. K., Loria, A. S., Hyndman, K. A., Jin, C., Clark, H., ... Pollock, J. S. (2018). Acute pressor response to psychosocial stress is dependent on endothelium-derived endothelin-1. Journal of the American Heart Association, 7(4), [e007863]. https://doi.org/10.1161/JAHA.117.007863

Acute pressor response to psychosocial stress is dependent on endothelium-derived endothelin-1. / Fox, Brandon M.; Becker, Bryan K.; Loria, Analia S.; Hyndman, Kelly A.; Jin, Chunhua; Clark, Hannah; Johns, Robin F; Yanagisawa, Masashi; Pollock, David M.; Pollock, Jennifer S.

In: Journal of the American Heart Association, Vol. 7, No. 4, e007863, 01.02.2018.

Research output: Contribution to journalArticle

Fox, BM, Becker, BK, Loria, AS, Hyndman, KA, Jin, C, Clark, H, Johns, RF, Yanagisawa, M, Pollock, DM & Pollock, JS 2018, 'Acute pressor response to psychosocial stress is dependent on endothelium-derived endothelin-1' Journal of the American Heart Association, vol. 7, no. 4, e007863. https://doi.org/10.1161/JAHA.117.007863
Fox, Brandon M. ; Becker, Bryan K. ; Loria, Analia S. ; Hyndman, Kelly A. ; Jin, Chunhua ; Clark, Hannah ; Johns, Robin F ; Yanagisawa, Masashi ; Pollock, David M. ; Pollock, Jennifer S. / Acute pressor response to psychosocial stress is dependent on endothelium-derived endothelin-1. In: Journal of the American Heart Association. 2018 ; Vol. 7, No. 4.
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abstract = "Background--Acute psychosocial stress provokes increases in circulating endothelin-1 (ET-1) levels in humans and animal models. However, key questions about the physiological function and cellular source of stress-induced ET-1 remain unanswered. We hypothesized that endothelium-derived ET-1 contributes to the acute pressor response to stress via activation of the endothelin A receptor. Methods and Results--Adult male vascular endothelium-specific ET-1 knockout mice and control mice that were homozygous for the floxed allele were exposed to acute psychosocial stress in the form of cage switch stress (CSS), with blood pressure measured by telemetry. An acute pressor response was elicited by CSS in both genotypes; however, this response was significantly blunted in vascular endothelium-specific ET-1 knockout mice compared with control mice that were homozygous for the floxed allele. In mice pretreated for 3 days with the endothelin A antagonist, ABT-627, or the dual endothelin A/B receptor antagonist, A-182086, the pressor response to CSS was similar between genotypes. CSS significantly increased plasma ET-1 levels in control mice that were homozygous for the floxed allele. CSS failed to elicit an increase in plasma ET-1 in vascular endothelium-specific ET-1 knockout mice. Telemetry frequency domain analyses suggested similar autonomic responses to stress between genotypes, and isolated resistance arteries demonstrated similar sensitivity to a1-adrenergic receptor-mediated vasoconstriction. Conclusions--These findings specify that acute stress-induced activation of endothelium-derived ET-1 and subsequent endothelin A receptor activation is a novel mediator of the blood pressure response to acute psychosocial stress.",
keywords = "Cage switch stress, Endothelin-1, Endothelium-derived factors, Psychosocial stress, Stress, Vascular endothelium-specific ET-1 knockout mice",
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AU - Becker, Bryan K.

AU - Loria, Analia S.

AU - Hyndman, Kelly A.

AU - Jin, Chunhua

AU - Clark, Hannah

AU - Johns, Robin F

AU - Yanagisawa, Masashi

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N2 - Background--Acute psychosocial stress provokes increases in circulating endothelin-1 (ET-1) levels in humans and animal models. However, key questions about the physiological function and cellular source of stress-induced ET-1 remain unanswered. We hypothesized that endothelium-derived ET-1 contributes to the acute pressor response to stress via activation of the endothelin A receptor. Methods and Results--Adult male vascular endothelium-specific ET-1 knockout mice and control mice that were homozygous for the floxed allele were exposed to acute psychosocial stress in the form of cage switch stress (CSS), with blood pressure measured by telemetry. An acute pressor response was elicited by CSS in both genotypes; however, this response was significantly blunted in vascular endothelium-specific ET-1 knockout mice compared with control mice that were homozygous for the floxed allele. In mice pretreated for 3 days with the endothelin A antagonist, ABT-627, or the dual endothelin A/B receptor antagonist, A-182086, the pressor response to CSS was similar between genotypes. CSS significantly increased plasma ET-1 levels in control mice that were homozygous for the floxed allele. CSS failed to elicit an increase in plasma ET-1 in vascular endothelium-specific ET-1 knockout mice. Telemetry frequency domain analyses suggested similar autonomic responses to stress between genotypes, and isolated resistance arteries demonstrated similar sensitivity to a1-adrenergic receptor-mediated vasoconstriction. Conclusions--These findings specify that acute stress-induced activation of endothelium-derived ET-1 and subsequent endothelin A receptor activation is a novel mediator of the blood pressure response to acute psychosocial stress.

AB - Background--Acute psychosocial stress provokes increases in circulating endothelin-1 (ET-1) levels in humans and animal models. However, key questions about the physiological function and cellular source of stress-induced ET-1 remain unanswered. We hypothesized that endothelium-derived ET-1 contributes to the acute pressor response to stress via activation of the endothelin A receptor. Methods and Results--Adult male vascular endothelium-specific ET-1 knockout mice and control mice that were homozygous for the floxed allele were exposed to acute psychosocial stress in the form of cage switch stress (CSS), with blood pressure measured by telemetry. An acute pressor response was elicited by CSS in both genotypes; however, this response was significantly blunted in vascular endothelium-specific ET-1 knockout mice compared with control mice that were homozygous for the floxed allele. In mice pretreated for 3 days with the endothelin A antagonist, ABT-627, or the dual endothelin A/B receptor antagonist, A-182086, the pressor response to CSS was similar between genotypes. CSS significantly increased plasma ET-1 levels in control mice that were homozygous for the floxed allele. CSS failed to elicit an increase in plasma ET-1 in vascular endothelium-specific ET-1 knockout mice. Telemetry frequency domain analyses suggested similar autonomic responses to stress between genotypes, and isolated resistance arteries demonstrated similar sensitivity to a1-adrenergic receptor-mediated vasoconstriction. Conclusions--These findings specify that acute stress-induced activation of endothelium-derived ET-1 and subsequent endothelin A receptor activation is a novel mediator of the blood pressure response to acute psychosocial stress.

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