Adenosine kinase deficiency in the brain results in maladaptive synaptic plasticity

Ursula S. Sandau, Mariana Colino-Oliveira, Abbie Jones, Bounmy Saleumvong, Shayla Q. Coffman, Long Liu, Catarina Miranda-Lourenço, Cátia Palminha, Vânia L. Batalha, Yiming Xu, Yuqing Huo, Maria J. Diógenes, Ana M. Sebastião, Detlev Boison

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Adenosine kinase (ADK) deficiency in human patients (OMIM:614300) disrupts the methionine cycle and triggers hypermethioninemia, hepatic encephalopathy, cognitive impairment, and seizures. To identify whether this neurological phenotype is intrinsically based on ADK deficiency in the brain or if it is secondary to liver dysfunction, we generated a mouse model with a brain-wide deletion of ADK by introducing a Nestin-Cre transgene into a line of conditional ADK deficient Adkfl/fl mice. These Adk brain mice developed a progressive stress-induced seizure phenotype associated with spontaneous convulsive seizures and profound deficits in hippocampus-dependent learning and memory. Pharmacological, biochemical, and electrophysiological studies suggest enhanced adenosine levels around syn-apses resulting in an enhanced adenosine A1 receptor (A1R)-dependent protective tone despite lower expression levels of the receptor. Theta-burst-induced LTP was enhanced in the mutants and this was dependent on adenosine A2A receptor (A2AR) and tropomyosin-related kinase B signaling, suggesting increased activation of these receptors in synaptic plasticity phenomena. Accordingly, reducing adenosine A2A receptor activity in Adk∆brain mice restored normal associative learning and contextual memory and attenuated seizure risk. We conclude that ADK deficiency in the brain triggers neuronal adaptation processes that lead to dysregulated synaptic plasticity, cognitive deficits, and increased seizure risk. Therefore, ADK mutations have an intrinsic effect on brain physiology and may present a genetic risk factor for the development of seizures and learning impairments. Furthermore, our data show that blocking A2AR activity therapeutically can attenuate neurological symptoms in ADK deficiency.

Original languageEnglish (US)
Pages (from-to)12117-12128
Number of pages12
JournalJournal of Neuroscience
Volume36
Issue number48
DOIs
StatePublished - Nov 30 2016

Fingerprint

Adenosine Kinase
Neuronal Plasticity
Seizures
Brain
Adenosine A2A Receptors
Learning
Genetic Databases
Phenotype
Adenosine A1 Receptors
Nestin
Hepatic Encephalopathy
Transgenes
Methionine
Adenosine
Liver Diseases
Hippocampus
Pharmacology
Mutation

Keywords

  • Adenosine kinase
  • Epilepsy
  • Gene mutation
  • Human genetic disorder
  • Learning and memory
  • Mouse model

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Sandau, U. S., Colino-Oliveira, M., Jones, A., Saleumvong, B., Coffman, S. Q., Liu, L., ... Boison, D. (2016). Adenosine kinase deficiency in the brain results in maladaptive synaptic plasticity. Journal of Neuroscience, 36(48), 12117-12128. https://doi.org/10.1523/JNEUROSCI.2146-16.2016

Adenosine kinase deficiency in the brain results in maladaptive synaptic plasticity. / Sandau, Ursula S.; Colino-Oliveira, Mariana; Jones, Abbie; Saleumvong, Bounmy; Coffman, Shayla Q.; Liu, Long; Miranda-Lourenço, Catarina; Palminha, Cátia; Batalha, Vânia L.; Xu, Yiming; Huo, Yuqing; Diógenes, Maria J.; Sebastião, Ana M.; Boison, Detlev.

In: Journal of Neuroscience, Vol. 36, No. 48, 30.11.2016, p. 12117-12128.

Research output: Contribution to journalArticle

Sandau, US, Colino-Oliveira, M, Jones, A, Saleumvong, B, Coffman, SQ, Liu, L, Miranda-Lourenço, C, Palminha, C, Batalha, VL, Xu, Y, Huo, Y, Diógenes, MJ, Sebastião, AM & Boison, D 2016, 'Adenosine kinase deficiency in the brain results in maladaptive synaptic plasticity', Journal of Neuroscience, vol. 36, no. 48, pp. 12117-12128. https://doi.org/10.1523/JNEUROSCI.2146-16.2016
Sandau US, Colino-Oliveira M, Jones A, Saleumvong B, Coffman SQ, Liu L et al. Adenosine kinase deficiency in the brain results in maladaptive synaptic plasticity. Journal of Neuroscience. 2016 Nov 30;36(48):12117-12128. https://doi.org/10.1523/JNEUROSCI.2146-16.2016
Sandau, Ursula S. ; Colino-Oliveira, Mariana ; Jones, Abbie ; Saleumvong, Bounmy ; Coffman, Shayla Q. ; Liu, Long ; Miranda-Lourenço, Catarina ; Palminha, Cátia ; Batalha, Vânia L. ; Xu, Yiming ; Huo, Yuqing ; Diógenes, Maria J. ; Sebastião, Ana M. ; Boison, Detlev. / Adenosine kinase deficiency in the brain results in maladaptive synaptic plasticity. In: Journal of Neuroscience. 2016 ; Vol. 36, No. 48. pp. 12117-12128.
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AU - Liu, Long

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AU - Palminha, Cátia

AU - Batalha, Vânia L.

AU - Xu, Yiming

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