Adenosine stimulates depolarization and rise in cytoplasmic [Ca 2+] in type I cells of rat carotid bodies

Fenglian Xu, Jianhua Xu, Frederick W. Tse, Amy Tse

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

During hypoxia, the level of adenosine in the carotid bodies increases as a result of ATP catabolism and adenosine efflux via adenosine transporters. Using Ca2+ imaging, we found that adenosine, acting via A2A receptors, triggered a rise in cytoplasmic [Ca2+] ([Ca 2+]i) in type I (glomus) cells of rat carotid bodies. The adenosine response could be mimicked by forskolin (but not its inactive analog), and could be abolished by the PKA inhibitor H89. Simultaneous measurements of membrane potential (perforated patch recording) and [Ca2+] i showed that the adenosine-mediated [Ca2+]i rise was accompanied by depolarization. Ni2+, a voltage-gated Ca 2+ channel (VGCC) blocker, abolished the adenosine-mediated [Ca 2+]i rise. Although adenosine was reported to inhibit a 4-aminopyridine (4-AP)-sensitive K+ current, 4-AP failed to trigger any [Ca2+]i rise, or to attenuate the adenosine response. In contrast, anandamide, an inhibitor of the TWIK-related acid-sensitive K +-1 (TASK-1) channels, triggered depolarization and [Ca 2+]i rise. The adenosine response was attenuated by anandamide but not by tetraethylammonium. Our results suggest that adenosine, acting via the adenylate cyclase and PKA pathways, inhibits the TASK-1 K + channels. This leads to depolarization and activation of Ca 2+ entry via VGCC. This excitatory action of adenosine on type I cells may contribute to the chemosensitivity of the carotid body during hypoxia.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Cell Physiology
Volume290
Issue number6
DOIs
StatePublished - Jun 1 2006
Externally publishedYes

Fingerprint

Carotid Body
Depolarization
Adenosine
Rats
Cells
4-Aminopyridine
Acids
Tetraethylammonium
Electric potential
Colforsin
Adenylyl Cyclases
Membrane Potentials
Adenosine Triphosphate
Chemical activation

Keywords

  • A receptor
  • cAMP
  • O sensing
  • Protein kinase A
  • TWIK-related acid-sensitive K channel

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Cell Biology
  • Physiology

Cite this

Adenosine stimulates depolarization and rise in cytoplasmic [Ca 2+] in type I cells of rat carotid bodies. / Xu, Fenglian; Xu, Jianhua; Tse, Frederick W.; Tse, Amy.

In: American Journal of Physiology - Cell Physiology, Vol. 290, No. 6, 01.06.2006.

Research output: Contribution to journalArticle

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AB - During hypoxia, the level of adenosine in the carotid bodies increases as a result of ATP catabolism and adenosine efflux via adenosine transporters. Using Ca2+ imaging, we found that adenosine, acting via A2A receptors, triggered a rise in cytoplasmic [Ca2+] ([Ca 2+]i) in type I (glomus) cells of rat carotid bodies. The adenosine response could be mimicked by forskolin (but not its inactive analog), and could be abolished by the PKA inhibitor H89. Simultaneous measurements of membrane potential (perforated patch recording) and [Ca2+] i showed that the adenosine-mediated [Ca2+]i rise was accompanied by depolarization. Ni2+, a voltage-gated Ca 2+ channel (VGCC) blocker, abolished the adenosine-mediated [Ca 2+]i rise. Although adenosine was reported to inhibit a 4-aminopyridine (4-AP)-sensitive K+ current, 4-AP failed to trigger any [Ca2+]i rise, or to attenuate the adenosine response. In contrast, anandamide, an inhibitor of the TWIK-related acid-sensitive K +-1 (TASK-1) channels, triggered depolarization and [Ca 2+]i rise. The adenosine response was attenuated by anandamide but not by tetraethylammonium. Our results suggest that adenosine, acting via the adenylate cyclase and PKA pathways, inhibits the TASK-1 K + channels. This leads to depolarization and activation of Ca 2+ entry via VGCC. This excitatory action of adenosine on type I cells may contribute to the chemosensitivity of the carotid body during hypoxia.

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