Abstract
OBJECTIVE: This work was conducted to investigate the expression of matrix metalloproteinase 9 (MMP 9) gene in cancer cells by fibronectin adhesion and the underlying mechanism of cell invasion. METHODS: Following adhesion of ovarian cancer cells A2780 to fibronectin, mRNA expression of MMP cells were assayed by reverse transcription-polymerase chain reaction (RT-PCR). MMP9 promoter was cloned from genomic DNA of HT1080 cells with PCR. The MMP-9-pGL2 reporter gene vector was constructed and then transiently transfected into A2780 cells. RESULTS: Adhesion induced the increase of cellular MMP9 mRNA content in A2780 cells, not affecting the expression of MMP2 or TIMP-1 gene. The stimulation was enhanced with the increase adhesion time. When the transfected cells were allowed to adhere and spread on FN-coated surface, the promoter activity of MMP9 gene was also enhanced dramatically. CONCLUSION: Cell-ECM adhesion may stimulate the expression of MMP9 gene through stimulating the promoter activity, thereby enhancing cancer cell invasion and metastasis.
Original language | English (US) |
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Pages (from-to) | 251-253 |
Number of pages | 3 |
Journal | Zhonghua zhong liu za zhi [Chinese journal of oncology] |
Volume | 24 |
Issue number | 1 |
DOIs | |
State | Published - Jan 1 2002 |
Externally published | Yes |
Keywords
- Adhesion fibronectin
- MRNA expression
- Matrix metalloproteinase
- Ovarian cancer
ASJC Scopus subject areas
- General Medicine