Adhesion to chondroitinase ABC treated dentin

Annalisa Mazzoni, David Henry Pashley, Alessandra Ruggeri, Francesca Vita, Mirella Falconi, Roberto Di Lenarda, Lorenzo Breschi

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Dentin bonding relies on complete resin impregnation throughout the demineralised hydrophilic collagen mesh. Chondroitin sulphate-glycosaminoglycans are claimed to regulate the three-dimensional arrangement of the dentin organic matrix and its hydrophilicity. The aim of this study was to investigate bond strength of two etch-and-rinse adhesives to chondroitinase ABC treated dentin. Human extracted molars were treated with chondroitinase ABC and a double labeling immunohistochemical technique was applied to reveal type I collagen and chondroitin 4/6 sulphate distribution under field emission in-lens scanning electron microscope. The immunohistochemical technique confirmed the effective removal of chondroitin 4/6 sulphate after the enzymatic treatment. Dentin surfaces exposed to chondroitinase ABC and untreated specimens prepared on untreated acid-etched dentin were bonded with Adper Scotchbond Multi-Purpose or Prime and Bond NT. Bonded specimens were submitted to microtensile testing and nanoleakage interfacial analysis under transmission electron microscope. Increased mean values of microtensile bond strength and reduced nanoleakage expression were found for both adhesives after chondroitinase ABC treatment of the dentin surface. Adper Scotchbond Multi-Purpose increased its bond strength about 28%, while bonding made with Prime and Bond NT almost doubled (92% increase) compared to untreated specimens. This study supports the hypothesis that adhesion can be enhanced by removal of chondroitin 4/6 sulphate and dermatan sulphate, probably due to a reduced amount of water content and enlarged interfibrillar spaces. Further studies should validate this hypothesis investigating the stability of chondroitin 4/6 and dermatan sulphate-depleted dentin bonded interface over time.

Original languageEnglish (US)
Pages (from-to)228-236
Number of pages9
JournalJournal of Biomedical Materials Research - Part B Applied Biomaterials
Volume86
Issue number1
DOIs
StatePublished - Jul 1 2008

Fingerprint

Chondroitin
Chondroitin ABC Lyase
Chondroitin Sulfates
Dentin
Adhesion
Dermatan Sulfate
Sulfates
Adhesives
Electron microscopes
Collagen
Hydrophilicity
Collagen Type I
Impregnation
Field emission
Labeling
Water content
Lenses
Resins
Electrons
Scanning

Keywords

  • Dental bonding systems
  • Dentin
  • Microtensile bond strength
  • Proteoglycans

ASJC Scopus subject areas

  • Biomaterials
  • Biomedical Engineering

Cite this

Mazzoni, A., Pashley, D. H., Ruggeri, A., Vita, F., Falconi, M., Di Lenarda, R., & Breschi, L. (2008). Adhesion to chondroitinase ABC treated dentin. Journal of Biomedical Materials Research - Part B Applied Biomaterials, 86(1), 228-236. https://doi.org/10.1002/jbm.b.31010

Adhesion to chondroitinase ABC treated dentin. / Mazzoni, Annalisa; Pashley, David Henry; Ruggeri, Alessandra; Vita, Francesca; Falconi, Mirella; Di Lenarda, Roberto; Breschi, Lorenzo.

In: Journal of Biomedical Materials Research - Part B Applied Biomaterials, Vol. 86, No. 1, 01.07.2008, p. 228-236.

Research output: Contribution to journalArticle

Mazzoni, A, Pashley, DH, Ruggeri, A, Vita, F, Falconi, M, Di Lenarda, R & Breschi, L 2008, 'Adhesion to chondroitinase ABC treated dentin', Journal of Biomedical Materials Research - Part B Applied Biomaterials, vol. 86, no. 1, pp. 228-236. https://doi.org/10.1002/jbm.b.31010
Mazzoni A, Pashley DH, Ruggeri A, Vita F, Falconi M, Di Lenarda R et al. Adhesion to chondroitinase ABC treated dentin. Journal of Biomedical Materials Research - Part B Applied Biomaterials. 2008 Jul 1;86(1):228-236. https://doi.org/10.1002/jbm.b.31010
Mazzoni, Annalisa ; Pashley, David Henry ; Ruggeri, Alessandra ; Vita, Francesca ; Falconi, Mirella ; Di Lenarda, Roberto ; Breschi, Lorenzo. / Adhesion to chondroitinase ABC treated dentin. In: Journal of Biomedical Materials Research - Part B Applied Biomaterials. 2008 ; Vol. 86, No. 1. pp. 228-236.
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