Abstract
Two experiments examined the effects of replacing high fat with low fat diets on adipocyte insulin sensitivity and response. Female Sprague-Dawley rats had free access to diets containing 21% (control), 61% (high fat) or 2% (low fat) of energy as fat. In the low fat diet a carbohydrate-based fat- mimetic carbohydrate replaced all but the essential fat present in the high fat diet. Insulin-stimulated glucose utilization by isolated adipocytes was measured after 10, 30 or 50 d. In a second study adipocyte insulin saturation curves were measured after 36 d. Rats fed the high fat diet for 30 d were insulin resistant and adipocyte basal and insulin-stimulated glucose utilization were depressed. The low fat diet initially stimulated glucose utilization of adipocytes but did not change insulin responsiveness. After 50 d there was no difference in glucose utilization between adipocytes from rats fed control and low fat diets. Insulin resistance in rats fed the high fat diet was associated with a nonsignificant reduction in insulin receptor number. These observations do not exclude the possibility of a post-receptor defect in glucose utilization.
Original language | English (US) |
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Pages (from-to) | 1802-1810 |
Number of pages | 9 |
Journal | Journal of Nutrition |
Volume | 122 |
Issue number | 9 |
DOIs | |
State | Published - 1992 |
Externally published | Yes |
Keywords
- adipocytes
- dietary fat
- fat-mimetic carbohydrate
- insulin response
- rats
ASJC Scopus subject areas
- Medicine (miscellaneous)
- Nutrition and Dietetics