Adipose-derived human stem/stromal cells: comparative organ specific mitochondrial bioenergy profiles

Alice S. Ferng, Katherine M. Marsh, Jamie M. Fleming, Renee F. Conway, David Schipper, Naing Bajaj, Alana M. Connell, Tia Pilikian, Kitsie Johnson, Ray Runyan, Stephen M. Black, John A. Szivek, Zain Khalpey

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Background: Adipose-derived stem/stromal cells (ASCs) isolated from the stromal vascular fraction are a source of mesenchymal stem cells that have been shown to be beneficial in many regenerative medicine applications. ASCs are an attractive source of stem cells in particular, due to their lack of immunogenicity. This study examines differences between mitochondrial bioenergetic profiles of ASCs isolated from adipose tissue of five peri-organ regions: pericardial, thymic, knee, shoulder, and abdomen. Results: Flow cytometry showed that the majority of each ASC population isolated from the adipose tissue of 12 donors, with an n = 3 for each tissue type, were positive for MSC markers CD90, CD73, and CD105, and negative for hematopoietic markers CD34, CD11B, CD19, and CD45. Bioenergetic profiles were obtained for ASCs with an n = 4 for each tissue type and graphed together for comparison. Mitochondrial stress tests provided the following measurements: basal respiration rate (measured as oxygen consumption rate [pmol O2/min], ATP production, proton leak, maximal respiration, respiratory control ratio, coupling efficiency, and non-mitochondrial respiration. Glycolytic stress tests provided the following measurements: basal glycolysis rate (measured as extracellular acidification rate [mpH/min]), glycolytic capacity, glycolytic reserve, and non-glycolytic acidification. Conclusions: The main goal of this manuscript was to provide baseline reference values for future experiments and to compare bioenergetic potentials of ASCs isolated from adipose tissue harvested from different anatomical locations. Through an investigation of mitochondrial respiration and glycolysis, it was demonstrated that bioenergetic profiles do not significantly differ by region due to depot-dependent and donor-dependent variability. Thus, although the physiological function, microenvironment and anatomical harvest site may directly affect the characteristics of ASCs isolated from different organ regions, the ultimate utility of ASCs remains independent of the anatomical harvest site.

Original languageEnglish (US)
Article number2057
JournalSpringerPlus
Volume5
Issue number1
DOIs
StatePublished - Dec 1 2016

Fingerprint

Stromal Cells
Stem Cells
Energy Metabolism
Adipose Tissue
Respiration
Glycolysis
Exercise Test
Regenerative Medicine
Respiratory Rate
Mesenchymal Stromal Cells
Oxygen Consumption
Abdomen
Blood Vessels
Protons
Knee
Flow Cytometry
Reference Values
Adenosine Triphosphate
Tissue Donors

Keywords

  • Adipose-derived stem/stromal cells
  • Bioenergetic profiling
  • Extracellular flux
  • Human adipose tissue
  • Mesenchymal stem cells
  • Mitochondrial bioenergetics
  • Stromal vascular fraction
  • Tissue engineering

ASJC Scopus subject areas

  • General

Cite this

Ferng, A. S., Marsh, K. M., Fleming, J. M., Conway, R. F., Schipper, D., Bajaj, N., ... Khalpey, Z. (2016). Adipose-derived human stem/stromal cells: comparative organ specific mitochondrial bioenergy profiles. SpringerPlus, 5(1), [2057]. https://doi.org/10.1186/s40064-016-3712-1

Adipose-derived human stem/stromal cells : comparative organ specific mitochondrial bioenergy profiles. / Ferng, Alice S.; Marsh, Katherine M.; Fleming, Jamie M.; Conway, Renee F.; Schipper, David; Bajaj, Naing; Connell, Alana M.; Pilikian, Tia; Johnson, Kitsie; Runyan, Ray; Black, Stephen M.; Szivek, John A.; Khalpey, Zain.

In: SpringerPlus, Vol. 5, No. 1, 2057, 01.12.2016.

Research output: Contribution to journalArticle

Ferng, AS, Marsh, KM, Fleming, JM, Conway, RF, Schipper, D, Bajaj, N, Connell, AM, Pilikian, T, Johnson, K, Runyan, R, Black, SM, Szivek, JA & Khalpey, Z 2016, 'Adipose-derived human stem/stromal cells: comparative organ specific mitochondrial bioenergy profiles', SpringerPlus, vol. 5, no. 1, 2057. https://doi.org/10.1186/s40064-016-3712-1
Ferng, Alice S. ; Marsh, Katherine M. ; Fleming, Jamie M. ; Conway, Renee F. ; Schipper, David ; Bajaj, Naing ; Connell, Alana M. ; Pilikian, Tia ; Johnson, Kitsie ; Runyan, Ray ; Black, Stephen M. ; Szivek, John A. ; Khalpey, Zain. / Adipose-derived human stem/stromal cells : comparative organ specific mitochondrial bioenergy profiles. In: SpringerPlus. 2016 ; Vol. 5, No. 1.
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abstract = "Background: Adipose-derived stem/stromal cells (ASCs) isolated from the stromal vascular fraction are a source of mesenchymal stem cells that have been shown to be beneficial in many regenerative medicine applications. ASCs are an attractive source of stem cells in particular, due to their lack of immunogenicity. This study examines differences between mitochondrial bioenergetic profiles of ASCs isolated from adipose tissue of five peri-organ regions: pericardial, thymic, knee, shoulder, and abdomen. Results: Flow cytometry showed that the majority of each ASC population isolated from the adipose tissue of 12 donors, with an n = 3 for each tissue type, were positive for MSC markers CD90, CD73, and CD105, and negative for hematopoietic markers CD34, CD11B, CD19, and CD45. Bioenergetic profiles were obtained for ASCs with an n = 4 for each tissue type and graphed together for comparison. Mitochondrial stress tests provided the following measurements: basal respiration rate (measured as oxygen consumption rate [pmol O2/min], ATP production, proton leak, maximal respiration, respiratory control ratio, coupling efficiency, and non-mitochondrial respiration. Glycolytic stress tests provided the following measurements: basal glycolysis rate (measured as extracellular acidification rate [mpH/min]), glycolytic capacity, glycolytic reserve, and non-glycolytic acidification. Conclusions: The main goal of this manuscript was to provide baseline reference values for future experiments and to compare bioenergetic potentials of ASCs isolated from adipose tissue harvested from different anatomical locations. Through an investigation of mitochondrial respiration and glycolysis, it was demonstrated that bioenergetic profiles do not significantly differ by region due to depot-dependent and donor-dependent variability. Thus, although the physiological function, microenvironment and anatomical harvest site may directly affect the characteristics of ASCs isolated from different organ regions, the ultimate utility of ASCs remains independent of the anatomical harvest site.",
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AU - Schipper, David

AU - Bajaj, Naing

AU - Connell, Alana M.

AU - Pilikian, Tia

AU - Johnson, Kitsie

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KW - Tissue engineering

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