ADP-ribosylation factor, a small GTP-dependent regulatory protein, stimulates phospholipase D activity

H. Alex Brown, Stephen Gutowski, Carolyn R. Moomaw, Clive A. Slaughter, Paul C. Sternwels

Research output: Contribution to journalArticle

788 Citations (Scopus)

Abstract

The hydrolysis of phosphatidylcholine by phospholipase D (PLD) results in the production of phosphatidic acid and choline. An assay that uses an exogenous substrate was developed to measure this activity in membranes and solubilized preparations from HL60 cells. A cytosolic factor markedly enhanced PLD activity in membranes and was essential for GTPγS-dependent stimulation of an enriched preparation of PLD. The factor was purified to homogeneity from bovine brain cytosol and identified as a member of the ADP-Ribosylation Factor (ARF) subfamily of small G proteins. Subsequently, recombinant myristoylated ARF1 was found to be a better activator of PLD activity than was the nonmyristoylated form. ARF proteins have been implicated recently as factors for regulation of intracellular vesicle traffic. The current finding suggests that PLD activity plays a prominent role in the action of ARF and that ARF may be a key component in the generation of second messengers via phospholipase D.

Original languageEnglish (US)
Pages (from-to)1137-1144
Number of pages8
JournalCell
Volume75
Issue number6
DOIs
StatePublished - Dec 17 1993
Externally publishedYes

Fingerprint

ADP-Ribosylation Factors
Phospholipase D
Guanosine Triphosphate
GTP-Binding Proteins
Proteins
Membranes
Phosphatidic Acids
Monomeric GTP-Binding Proteins
HL-60 Cells
Second Messenger Systems
Choline
Phosphatidylcholines
Cytosol
Hydrolysis
Assays
Brain
Substrates

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

ADP-ribosylation factor, a small GTP-dependent regulatory protein, stimulates phospholipase D activity. / Brown, H. Alex; Gutowski, Stephen; Moomaw, Carolyn R.; Slaughter, Clive A.; Sternwels, Paul C.

In: Cell, Vol. 75, No. 6, 17.12.1993, p. 1137-1144.

Research output: Contribution to journalArticle

Brown, H. Alex ; Gutowski, Stephen ; Moomaw, Carolyn R. ; Slaughter, Clive A. ; Sternwels, Paul C. / ADP-ribosylation factor, a small GTP-dependent regulatory protein, stimulates phospholipase D activity. In: Cell. 1993 ; Vol. 75, No. 6. pp. 1137-1144.
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