Advanced in vitro approach to study neurovascular coupling mechanisms in the brain microcirculation

Ki Jung Kim, Jessica A. Filosa

Research output: Contribution to journalArticle

21 Scopus citations

Abstract

An understanding of the signalling events underlying neurovascular coupling mechanisms in the brain is a crucial step in the development of novel therapeutic approaches for the treatment of cerebrovascular-associated disorders. In this study we present an enhanced in vitro brain slice preparation from male Wistar rat cortical slices that incorporates haemodynamic variables (flow and pressure) into parenchymal arterioles resulting in the development of myogenic tone (28% from maximum dilatation). Moreover, we characterized flow-induced vascular responses, resulting in various degrees of vasoconstrictions and the response to 10 mm K + or astrocytic activation with the mGluR agonist, t-ACPD (100 μm), resulting in vasodilatations of 33.6 ± 4.7% and 38.6 ± 4.6%, respectively. Using fluorescence recovery, we determined perfusate velocity to calculate diameter changes under different experimental pH conditions. Using this approach, we demonstrate no significant differences between diameter changes measured using video microscopy or predicted from the velocity values obtained using fluorescence recovery after photobleaching. The model is further validated by demonstrating our ability to cannulate arterioles in two brain regions (cortex and supraoptic nucleus of the hypothalamus). Altogether, we believe this is the first study demonstrating successful cannulation and perfusion of parenchymal arterioles while monitoring/estimating luminal diameter and pressure under conditions where flow rates are controlled.

Original languageEnglish (US)
Pages (from-to)1757-1770
Number of pages14
JournalJournal of Physiology
Volume590
Issue number7
DOIs
StatePublished - Apr 1 2012

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ASJC Scopus subject areas

  • Physiology

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