Age as an independent risk factor for severity of experimental choroidal neovascularization

Diego Gabriel Espinosa Heidmann, Ivan Suner, Eleut P. Hernandez, William D. Frazier, Karl G. Csaky, Scott W. Cousins

Research output: Contribution to journalArticle

62 Citations (Scopus)

Abstract

PURPOSE. For many vascular diseases, aging appears to be an independent risk factor for severity of vascular complications, and blood vessels of aged individuals often demonstrate exaggerated repair responses to injury. This study was undertaken to determine the influence of aging on the severity of neovascularization in a mouse model of laser-induced choroidal neovascularization (CNV). METHODS. CNV was induced in young (2-month-old) and aged (16-month-old) C57BL/6 mice by making four separate choroidal burns in each eye with a diode red laser (650 nm). At 1, 2, and 4 weeks, the left eyes were removed for histopathology, and the right eyes were removed for flatmount analysis of CNV surface area, vascularity, and cellularity. RESULTS. Aged mice demonstrated a much larger area of CNV than did young mice (3.81 ± 1.28 vs. 1.36 ± 0.99 disc areas, P < 0.001) at 2 weeks, when the lesions showed maximum growth. Aged mice also demonstrated higher ratios for vascularity and cellularity of the CNV (1.34 ± 0.06 vs. 1.03 ± 0.11, P < 0.0001 and 4.06 ± 1.19 vs. 1.91 ± 0.81, P < 0.002 at 2 weeks, respectively). Histopathology revealed that CNV in older eyes was larger, thicker, and more cellular than in young eyes. CONCLUSIONS. In mice, age is associated with more severe CNV, defined as larger surface area, greater vascularity, and greater cellularity. Age-related systemic susceptibility factors, independent of local changes in the retina, may contribute to the greater severity of CNV in older than in younger individuals.

Original languageEnglish (US)
Pages (from-to)1567-1573
Number of pages7
JournalInvestigative Ophthalmology and Visual Science
Volume43
Issue number5
StatePublished - May 13 2002

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Choroidal Neovascularization
Blood Vessels
Semiconductor Lasers
Burns
Inbred C57BL Mouse
Vascular Diseases
Retina
Lasers
Wounds and Injuries
Growth

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

Cite this

Espinosa Heidmann, D. G., Suner, I., Hernandez, E. P., Frazier, W. D., Csaky, K. G., & Cousins, S. W. (2002). Age as an independent risk factor for severity of experimental choroidal neovascularization. Investigative Ophthalmology and Visual Science, 43(5), 1567-1573.

Age as an independent risk factor for severity of experimental choroidal neovascularization. / Espinosa Heidmann, Diego Gabriel; Suner, Ivan; Hernandez, Eleut P.; Frazier, William D.; Csaky, Karl G.; Cousins, Scott W.

In: Investigative Ophthalmology and Visual Science, Vol. 43, No. 5, 13.05.2002, p. 1567-1573.

Research output: Contribution to journalArticle

Espinosa Heidmann, DG, Suner, I, Hernandez, EP, Frazier, WD, Csaky, KG & Cousins, SW 2002, 'Age as an independent risk factor for severity of experimental choroidal neovascularization', Investigative Ophthalmology and Visual Science, vol. 43, no. 5, pp. 1567-1573.
Espinosa Heidmann, Diego Gabriel ; Suner, Ivan ; Hernandez, Eleut P. ; Frazier, William D. ; Csaky, Karl G. ; Cousins, Scott W. / Age as an independent risk factor for severity of experimental choroidal neovascularization. In: Investigative Ophthalmology and Visual Science. 2002 ; Vol. 43, No. 5. pp. 1567-1573.
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AU - Suner, Ivan

AU - Hernandez, Eleut P.

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AU - Csaky, Karl G.

AU - Cousins, Scott W.

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AB - PURPOSE. For many vascular diseases, aging appears to be an independent risk factor for severity of vascular complications, and blood vessels of aged individuals often demonstrate exaggerated repair responses to injury. This study was undertaken to determine the influence of aging on the severity of neovascularization in a mouse model of laser-induced choroidal neovascularization (CNV). METHODS. CNV was induced in young (2-month-old) and aged (16-month-old) C57BL/6 mice by making four separate choroidal burns in each eye with a diode red laser (650 nm). At 1, 2, and 4 weeks, the left eyes were removed for histopathology, and the right eyes were removed for flatmount analysis of CNV surface area, vascularity, and cellularity. RESULTS. Aged mice demonstrated a much larger area of CNV than did young mice (3.81 ± 1.28 vs. 1.36 ± 0.99 disc areas, P < 0.001) at 2 weeks, when the lesions showed maximum growth. Aged mice also demonstrated higher ratios for vascularity and cellularity of the CNV (1.34 ± 0.06 vs. 1.03 ± 0.11, P < 0.0001 and 4.06 ± 1.19 vs. 1.91 ± 0.81, P < 0.002 at 2 weeks, respectively). Histopathology revealed that CNV in older eyes was larger, thicker, and more cellular than in young eyes. CONCLUSIONS. In mice, age is associated with more severe CNV, defined as larger surface area, greater vascularity, and greater cellularity. Age-related systemic susceptibility factors, independent of local changes in the retina, may contribute to the greater severity of CNV in older than in younger individuals.

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