Age-related alterations in NOS and oxidative stress in mesenteric arteries from male and female rats

Jennifer C Sullivan, Elizabeth Dabbs Loomis, Matthew Collins, John D. Imig, Edward W. Inscho, Jennifer S. Pollock

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Epidemiological evidence suggests that advancing age affects the cardiovascular system of men and women differently. The purpose of this study was to determine whether the effects of aging on nitric oxide synthase (NOS), oxidative stress, and vascular function are different in males and females. Mesenteric arteries from young (3 mo) and old (24 mo) male and female Fischer 344/Brown Norway rats were studied. Western blot analysis and NOS activity were performed on the homogenized mesenteric arterial bed separated into cytosolic and membrane-associated fractions. Plasma 8-isoprostane measurements assessed oxidative stress. Vascular reactivity was determined by using a wire myograph in the absence and presence of a NOS inhibitor, Nω-nitro-L- arginine, to examine endothelial function and basal and stimulated nitric oxide release. In additional arteries, reactivity was performed in the presence of polyethylene glycol-SOD to assess the impact of superoxide on vascular function. Among females, aging was associated with a decline in membrane-associated NOS activity and membrane-associated NOS III protein expression. Advancing age in males was associated with increased cytosolic NOS III protein expression. Among both males and females, advancing age resulted in increased oxidative stress. Vascular function was maintained with age in arteries from both males and females, and there was no difference in either basal or stimulated nitric oxide release with age. Despite sex-specific effects of advancing age on the NOS system and increases in markers of oxidative stress, vascular function is maintained in mesenteric arteries from aged Fischer 344/Brown Norway rats. These data suggest that age-related alterations in the resistance vasculature are complex and likely involve multiple compensating vasoactive pathways.

Original languageEnglish (US)
Pages (from-to)1268-1274
Number of pages7
JournalJournal of Applied Physiology
Volume97
Issue number4
DOIs
StatePublished - Oct 1 2004

Fingerprint

Mesenteric Arteries
Nitric Oxide Synthase
Oxidative Stress
Blood Vessels
8-epi-prostaglandin F2alpha
Membranes
Nitric Oxide
Arteries
Cardiovascular System
Superoxides
Arginine
Proteins
Western Blotting

Keywords

  • Aging
  • Nitric oxide
  • Oxygen radicals
  • Vascular reactivity

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)

Cite this

Age-related alterations in NOS and oxidative stress in mesenteric arteries from male and female rats. / Sullivan, Jennifer C; Loomis, Elizabeth Dabbs; Collins, Matthew; Imig, John D.; Inscho, Edward W.; Pollock, Jennifer S.

In: Journal of Applied Physiology, Vol. 97, No. 4, 01.10.2004, p. 1268-1274.

Research output: Contribution to journalArticle

Sullivan, Jennifer C ; Loomis, Elizabeth Dabbs ; Collins, Matthew ; Imig, John D. ; Inscho, Edward W. ; Pollock, Jennifer S. / Age-related alterations in NOS and oxidative stress in mesenteric arteries from male and female rats. In: Journal of Applied Physiology. 2004 ; Vol. 97, No. 4. pp. 1268-1274.
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