Age-related loss of muscle mass and bone strength in mice is associated with a decline in physical activity and serum leptin

Mark W. Hamrick, Ke Hong Ding, Catherine Pennington, Yuh J. Chao, Yii Der Wu, Boyd Howard, David Immel, Cesario Borlongan, Paul L. McNeil, Wendy B. Bollag, Walton W. Curl, Jack Yu, Carlos M. Isales

Research output: Contribution to journalArticle

91 Citations (Scopus)

Abstract

The mechanisms underlying age-related loss of muscle and bone tissue are poorly understood but are thought to involve changes in sex hormone status, physical activity, and circulating levels of inflammatory cytokines. This study attempts to develop an animal model useful for evaluating these mechanisms in vivo. Male C57BL/6 mice were included for study at 3, 6, 12, 18, 24, and 29 months of age. Endocortical mineralizing surface, serum leptin, body weight, and percentage of body fat all increased between 6 and 12 months of age as activity level declined. Serum levels of the inflammatory marker IL-6 increased significantly after 12 months of age, following the observed increase in body weight and percent body fat. Hindlimb muscle mass declined significantly between 18 and 24 months of age, both absolutely and relative to total body mass, with a further decline (∼15%) between 24 and 29 months. Loss of muscle mass after 18 months of age was accompanied by a significant increase in bone resorption, as indicated by serum pyridinoline cross-links, and a significant decrease in fat mass, serum leptin, bone strength, bone mineral density, and vertical cage activity. No significant changes in serum testosterone with aging were detected in the mice, as levels were essentially constant between 6 and 29 months. Our data show that mice lose a significant amount of muscle and bone tissue with age, and this loss of musculoskeletal tissue is accompanied by a drop in serum leptin and preceded by a significant decrease in physical activity.

Original languageEnglish (US)
Pages (from-to)845-853
Number of pages9
JournalBone
Volume39
Issue number4
DOIs
StatePublished - Oct 1 2006

Fingerprint

Leptin
Bone and Bones
Muscles
Serum
Adipose Tissue
Body Weight
Gonadal Steroid Hormones
Hindlimb
Bone Resorption
Inbred C57BL Mouse
Bone Density
Testosterone
Interleukin-6
Animal Models
Fats
Cytokines

Keywords

  • Aging
  • Bone loss
  • Bone mineral density
  • IL-6
  • Sarcopenia

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Physiology
  • Histology

Cite this

Age-related loss of muscle mass and bone strength in mice is associated with a decline in physical activity and serum leptin. / Hamrick, Mark W.; Ding, Ke Hong; Pennington, Catherine; Chao, Yuh J.; Wu, Yii Der; Howard, Boyd; Immel, David; Borlongan, Cesario; McNeil, Paul L.; Bollag, Wendy B.; Curl, Walton W.; Yu, Jack; Isales, Carlos M.

In: Bone, Vol. 39, No. 4, 01.10.2006, p. 845-853.

Research output: Contribution to journalArticle

Hamrick, Mark W. ; Ding, Ke Hong ; Pennington, Catherine ; Chao, Yuh J. ; Wu, Yii Der ; Howard, Boyd ; Immel, David ; Borlongan, Cesario ; McNeil, Paul L. ; Bollag, Wendy B. ; Curl, Walton W. ; Yu, Jack ; Isales, Carlos M. / Age-related loss of muscle mass and bone strength in mice is associated with a decline in physical activity and serum leptin. In: Bone. 2006 ; Vol. 39, No. 4. pp. 845-853.
@article{904538980c564554aaf256295227a707,
title = "Age-related loss of muscle mass and bone strength in mice is associated with a decline in physical activity and serum leptin",
abstract = "The mechanisms underlying age-related loss of muscle and bone tissue are poorly understood but are thought to involve changes in sex hormone status, physical activity, and circulating levels of inflammatory cytokines. This study attempts to develop an animal model useful for evaluating these mechanisms in vivo. Male C57BL/6 mice were included for study at 3, 6, 12, 18, 24, and 29 months of age. Endocortical mineralizing surface, serum leptin, body weight, and percentage of body fat all increased between 6 and 12 months of age as activity level declined. Serum levels of the inflammatory marker IL-6 increased significantly after 12 months of age, following the observed increase in body weight and percent body fat. Hindlimb muscle mass declined significantly between 18 and 24 months of age, both absolutely and relative to total body mass, with a further decline (∼15{\%}) between 24 and 29 months. Loss of muscle mass after 18 months of age was accompanied by a significant increase in bone resorption, as indicated by serum pyridinoline cross-links, and a significant decrease in fat mass, serum leptin, bone strength, bone mineral density, and vertical cage activity. No significant changes in serum testosterone with aging were detected in the mice, as levels were essentially constant between 6 and 29 months. Our data show that mice lose a significant amount of muscle and bone tissue with age, and this loss of musculoskeletal tissue is accompanied by a drop in serum leptin and preceded by a significant decrease in physical activity.",
keywords = "Aging, Bone loss, Bone mineral density, IL-6, Sarcopenia",
author = "Hamrick, {Mark W.} and Ding, {Ke Hong} and Catherine Pennington and Chao, {Yuh J.} and Wu, {Yii Der} and Boyd Howard and David Immel and Cesario Borlongan and McNeil, {Paul L.} and Bollag, {Wendy B.} and Curl, {Walton W.} and Jack Yu and Isales, {Carlos M.}",
year = "2006",
month = "10",
day = "1",
doi = "10.1016/j.bone.2006.04.011",
language = "English (US)",
volume = "39",
pages = "845--853",
journal = "Bone",
issn = "8756-3282",
publisher = "Elsevier Inc.",
number = "4",

}

TY - JOUR

T1 - Age-related loss of muscle mass and bone strength in mice is associated with a decline in physical activity and serum leptin

AU - Hamrick, Mark W.

AU - Ding, Ke Hong

AU - Pennington, Catherine

AU - Chao, Yuh J.

AU - Wu, Yii Der

AU - Howard, Boyd

AU - Immel, David

AU - Borlongan, Cesario

AU - McNeil, Paul L.

AU - Bollag, Wendy B.

AU - Curl, Walton W.

AU - Yu, Jack

AU - Isales, Carlos M.

PY - 2006/10/1

Y1 - 2006/10/1

N2 - The mechanisms underlying age-related loss of muscle and bone tissue are poorly understood but are thought to involve changes in sex hormone status, physical activity, and circulating levels of inflammatory cytokines. This study attempts to develop an animal model useful for evaluating these mechanisms in vivo. Male C57BL/6 mice were included for study at 3, 6, 12, 18, 24, and 29 months of age. Endocortical mineralizing surface, serum leptin, body weight, and percentage of body fat all increased between 6 and 12 months of age as activity level declined. Serum levels of the inflammatory marker IL-6 increased significantly after 12 months of age, following the observed increase in body weight and percent body fat. Hindlimb muscle mass declined significantly between 18 and 24 months of age, both absolutely and relative to total body mass, with a further decline (∼15%) between 24 and 29 months. Loss of muscle mass after 18 months of age was accompanied by a significant increase in bone resorption, as indicated by serum pyridinoline cross-links, and a significant decrease in fat mass, serum leptin, bone strength, bone mineral density, and vertical cage activity. No significant changes in serum testosterone with aging were detected in the mice, as levels were essentially constant between 6 and 29 months. Our data show that mice lose a significant amount of muscle and bone tissue with age, and this loss of musculoskeletal tissue is accompanied by a drop in serum leptin and preceded by a significant decrease in physical activity.

AB - The mechanisms underlying age-related loss of muscle and bone tissue are poorly understood but are thought to involve changes in sex hormone status, physical activity, and circulating levels of inflammatory cytokines. This study attempts to develop an animal model useful for evaluating these mechanisms in vivo. Male C57BL/6 mice were included for study at 3, 6, 12, 18, 24, and 29 months of age. Endocortical mineralizing surface, serum leptin, body weight, and percentage of body fat all increased between 6 and 12 months of age as activity level declined. Serum levels of the inflammatory marker IL-6 increased significantly after 12 months of age, following the observed increase in body weight and percent body fat. Hindlimb muscle mass declined significantly between 18 and 24 months of age, both absolutely and relative to total body mass, with a further decline (∼15%) between 24 and 29 months. Loss of muscle mass after 18 months of age was accompanied by a significant increase in bone resorption, as indicated by serum pyridinoline cross-links, and a significant decrease in fat mass, serum leptin, bone strength, bone mineral density, and vertical cage activity. No significant changes in serum testosterone with aging were detected in the mice, as levels were essentially constant between 6 and 29 months. Our data show that mice lose a significant amount of muscle and bone tissue with age, and this loss of musculoskeletal tissue is accompanied by a drop in serum leptin and preceded by a significant decrease in physical activity.

KW - Aging

KW - Bone loss

KW - Bone mineral density

KW - IL-6

KW - Sarcopenia

UR - http://www.scopus.com/inward/record.url?scp=33748193352&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33748193352&partnerID=8YFLogxK

U2 - 10.1016/j.bone.2006.04.011

DO - 10.1016/j.bone.2006.04.011

M3 - Article

C2 - 16750436

AN - SCOPUS:33748193352

VL - 39

SP - 845

EP - 853

JO - Bone

JF - Bone

SN - 8756-3282

IS - 4

ER -