Abstract
We measured the extent of complement activation and neutrophil mobilization after eccentric exercise to determine whether these responses were age dependent and whether they were associated with changes in plasma creatine kinase (CK), a marker for muscle membrane integrity. Repeated forced lengthening of a muscle as it develops tension causes immediate ultrastructural damage to sarcomeres, followed by delayed-onset muscle soreness and release of myocellular enzymes. This can be accomplished in quadriceps muscles by running downhill or by resisting bicycle pedals driven backward by a motor. Twelve older (61-72 yr) and 9 younger (20-32 yr) subjects performed one of these activities for 45 min at an intensity of 78 ± 2% of maximum heart rate. For all subjects, a median increase of 21% in plasma des-Arg-C3a levels occurred immediately after the protocol, circulating neutrophils increased 66 ± 10% by 4-6 h, and plasma CK increased 135 ± 25% by 24 h. The peak increases in neutrophils correlated with the peak increases in des-Arg-C3a (rho = 0.662, P = 0.006), and the peak increases in CK correlated with the rise in neutrophils (rho = 0.523, P = 0.027). The increases in neutrophils and plasma CK were significantly smaller in the older subjects (P < 0.05). The results indicate that increased concentrations of circulating CK after muscle injury are associated with a sequential cascade of inflammatory mediators. Furthermore, neutrophil mobilization, but not complement activation, was diminished in older subjects in response to this stress.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 2616-2620 |
| Number of pages | 5 |
| Journal | Journal of Applied Physiology |
| Volume | 76 |
| Issue number | 6 |
| DOIs | |
| State | Published - Jan 1 1994 |
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Keywords
- creatine kinase
- eccentric exercise
- muscle damage
ASJC Scopus subject areas
- Physiology
- Physiology (medical)
Cite this
Aging and stress-induced changes in complement activation and neutrophil mobilization. / Cannon, Joseph Gerard; Fiatarone, M. A.; Fielding, R. A.; Evans, W. J.
In: Journal of Applied Physiology, Vol. 76, No. 6, 01.01.1994, p. 2616-2620.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Aging and stress-induced changes in complement activation and neutrophil mobilization
AU - Cannon, Joseph Gerard
AU - Fiatarone, M. A.
AU - Fielding, R. A.
AU - Evans, W. J.
PY - 1994/1/1
Y1 - 1994/1/1
N2 - We measured the extent of complement activation and neutrophil mobilization after eccentric exercise to determine whether these responses were age dependent and whether they were associated with changes in plasma creatine kinase (CK), a marker for muscle membrane integrity. Repeated forced lengthening of a muscle as it develops tension causes immediate ultrastructural damage to sarcomeres, followed by delayed-onset muscle soreness and release of myocellular enzymes. This can be accomplished in quadriceps muscles by running downhill or by resisting bicycle pedals driven backward by a motor. Twelve older (61-72 yr) and 9 younger (20-32 yr) subjects performed one of these activities for 45 min at an intensity of 78 ± 2% of maximum heart rate. For all subjects, a median increase of 21% in plasma des-Arg-C3a levels occurred immediately after the protocol, circulating neutrophils increased 66 ± 10% by 4-6 h, and plasma CK increased 135 ± 25% by 24 h. The peak increases in neutrophils correlated with the peak increases in des-Arg-C3a (rho = 0.662, P = 0.006), and the peak increases in CK correlated with the rise in neutrophils (rho = 0.523, P = 0.027). The increases in neutrophils and plasma CK were significantly smaller in the older subjects (P < 0.05). The results indicate that increased concentrations of circulating CK after muscle injury are associated with a sequential cascade of inflammatory mediators. Furthermore, neutrophil mobilization, but not complement activation, was diminished in older subjects in response to this stress.
AB - We measured the extent of complement activation and neutrophil mobilization after eccentric exercise to determine whether these responses were age dependent and whether they were associated with changes in plasma creatine kinase (CK), a marker for muscle membrane integrity. Repeated forced lengthening of a muscle as it develops tension causes immediate ultrastructural damage to sarcomeres, followed by delayed-onset muscle soreness and release of myocellular enzymes. This can be accomplished in quadriceps muscles by running downhill or by resisting bicycle pedals driven backward by a motor. Twelve older (61-72 yr) and 9 younger (20-32 yr) subjects performed one of these activities for 45 min at an intensity of 78 ± 2% of maximum heart rate. For all subjects, a median increase of 21% in plasma des-Arg-C3a levels occurred immediately after the protocol, circulating neutrophils increased 66 ± 10% by 4-6 h, and plasma CK increased 135 ± 25% by 24 h. The peak increases in neutrophils correlated with the peak increases in des-Arg-C3a (rho = 0.662, P = 0.006), and the peak increases in CK correlated with the rise in neutrophils (rho = 0.523, P = 0.027). The increases in neutrophils and plasma CK were significantly smaller in the older subjects (P < 0.05). The results indicate that increased concentrations of circulating CK after muscle injury are associated with a sequential cascade of inflammatory mediators. Furthermore, neutrophil mobilization, but not complement activation, was diminished in older subjects in response to this stress.
KW - creatine kinase
KW - eccentric exercise
KW - muscle damage
UR - http://www.scopus.com/inward/record.url?scp=0028332960&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0028332960&partnerID=8YFLogxK
U2 - 10.1152/jappl.1994.76.6.2616
DO - 10.1152/jappl.1994.76.6.2616
M3 - Article
C2 - 7928891
AN - SCOPUS:0028332960
VL - 76
SP - 2616
EP - 2620
JO - Journal of Applied Physiology Respiratory Environmental and Exercise Physiology
JF - Journal of Applied Physiology Respiratory Environmental and Exercise Physiology
SN - 8750-7587
IS - 6
ER -