AIDP and CIDP having specific antibodies to the carbohydrate epitope (-NeuAcα2-8NeuAcα2-3Galβ1-4Glc-) of gangliosides

Seigo Usuki, Juan Sanchez, Toshio Ariga, Iku Utsunomiya, Kyoji Taguchi, Michael H. Rivner, Robert K. Yu

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Anti-ganglioside antibodies were investigated in plasma exchange solutions (PEs) from two patients with acute and chronic inflammatory demyelinating neuropathies (AIDP and CIDP). Both cases show markedly elevated antibody titers against the lacto-series gangliosides, GM3, GD3, and GT3. In the CIDP patient, the IgG antibody titer to GD3 was remarkably elevated (titer, 1:10,000), indicating maximal avidity to the tetrasaccharide epitope (-NeuAcα2- 8NeuAcα2-3Galβ1-4Glc-). There were also activities toward GM4 and GM2 with the affinity higher to GM4 than to GM2, indicating that the antibody activity was not highly specific. In contrast, the antibody activities in the AIDP patient showed similar avidity to GM3, GD3, and GT3. These two patients are very rare cases that have not previously encountered in GBS. The effects on co-cultured cells of rat spinal cord and muscle differed according to which PE was used. PE from the AIDP patient produced an inhibitory effect (reduction to 26.8%) on the spontaneous muscle action potential of the neuromuscular junction (NMJ), but the PE from the CIDP patient did not. Thus, in AIDP, the common epitope of GM3, GD3, or GT3 may be shared with certain antigens localized in the peripheral nervous system (PNS) and may participate in a component of conduction-related molecules in the NMJ. High titers of anti-GD3 antibody and the distortion of antibody recognition found in CIDP seem to have no immediate effect on electrophysiologic function in the PNS.

Original languageEnglish (US)
Pages (from-to)37-44
Number of pages8
JournalJournal of the Neurological Sciences
Volume232
Issue number1-2
DOIs
StatePublished - May 15 2005

Fingerprint

Chronic Inflammatory Demyelinating Polyradiculoneuropathy
Gangliosides
Epitopes
Carbohydrates
Antibodies
Neuromuscular Junction
Peripheral Nervous System
Anti-Idiotypic Antibodies
G(M3) Ganglioside
Muscles
Plasma Exchange
Action Potentials
Cultured Cells
Spinal Cord
Immunoglobulin G
Antigens

Keywords

  • AIDP
  • Anti-ganglioside antibody
  • CIDP
  • GD3
  • Guillain-Barré syndrome

ASJC Scopus subject areas

  • Neurology
  • Clinical Neurology

Cite this

AIDP and CIDP having specific antibodies to the carbohydrate epitope (-NeuAcα2-8NeuAcα2-3Galβ1-4Glc-) of gangliosides. / Usuki, Seigo; Sanchez, Juan; Ariga, Toshio; Utsunomiya, Iku; Taguchi, Kyoji; Rivner, Michael H.; Yu, Robert K.

In: Journal of the Neurological Sciences, Vol. 232, No. 1-2, 15.05.2005, p. 37-44.

Research output: Contribution to journalArticle

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title = "AIDP and CIDP having specific antibodies to the carbohydrate epitope (-NeuAcα2-8NeuAcα2-3Galβ1-4Glc-) of gangliosides",
abstract = "Anti-ganglioside antibodies were investigated in plasma exchange solutions (PEs) from two patients with acute and chronic inflammatory demyelinating neuropathies (AIDP and CIDP). Both cases show markedly elevated antibody titers against the lacto-series gangliosides, GM3, GD3, and GT3. In the CIDP patient, the IgG antibody titer to GD3 was remarkably elevated (titer, 1:10,000), indicating maximal avidity to the tetrasaccharide epitope (-NeuAcα2- 8NeuAcα2-3Galβ1-4Glc-). There were also activities toward GM4 and GM2 with the affinity higher to GM4 than to GM2, indicating that the antibody activity was not highly specific. In contrast, the antibody activities in the AIDP patient showed similar avidity to GM3, GD3, and GT3. These two patients are very rare cases that have not previously encountered in GBS. The effects on co-cultured cells of rat spinal cord and muscle differed according to which PE was used. PE from the AIDP patient produced an inhibitory effect (reduction to 26.8{\%}) on the spontaneous muscle action potential of the neuromuscular junction (NMJ), but the PE from the CIDP patient did not. Thus, in AIDP, the common epitope of GM3, GD3, or GT3 may be shared with certain antigens localized in the peripheral nervous system (PNS) and may participate in a component of conduction-related molecules in the NMJ. High titers of anti-GD3 antibody and the distortion of antibody recognition found in CIDP seem to have no immediate effect on electrophysiologic function in the PNS.",
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AU - Sanchez, Juan

AU - Ariga, Toshio

AU - Utsunomiya, Iku

AU - Taguchi, Kyoji

AU - Rivner, Michael H.

AU - Yu, Robert K.

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