Alpha-4 integrins and VCAM-1, but not MAdCAM-1, are essential for recruitment of mast cell progenitors to the inflamed lung

J. Pablo Abonia, Jenny Hallgren, Tatiana Jones, Tong Shi, Yuhui Xu, Pandelakis Koni, Richard A. Flavell, Joshua A. Boyce, K. Frank Austen, Michael F. Gurish

Research output: Contribution to journalArticle

93 Citations (Scopus)

Abstract

Normal mouse lungs lack appreciable numbers of mast cells (MCs) or MC progenitors (MCp's), yet the appearance of mature MCs in the tracheobronchial epithelial surface is a characteristic of allergic, T-cell-dependent pulmonary inflammation. We hypothesized that pulmonary inflammation would recruit MCp's to inflamed lungs and that this recruitment would be regulated by distinct adhesion pathways. Ovalbumin-sensitized and challenged mice had a greater than 28-fold increase in the number of MCp's in the lungs. In mice lacking endothelial vascular cell adhesion molecule 1 (VCAM-1) and in wild-type mice administered blocking monoclonal antibody (mAb) to VCAM-1 but not to mucosal addressin CAM-1 (MadCAM-1), recruitment of MCp's to the inflamed lung was reduced by greater than 75%. Analysis of the integrin receptors for VCAM-1 showed that in β7 integrin-deficient mice, recruitment was reduced 73% relative to wild-type controls, and in either BALB/c or C57BL/6 mice, mAb blocking of α4, β1, or β7 integrins inhibited the recruitment of MCp's to the inflamed lung. Thus, VCAM-1 interactions with both α4β1 and α4β7 integrins are essential for the recruitment and expansion of the MCp populations in the lung during antigen-induced pulmonary inflammation. Furthermore, the MCp is currently unique among inflammatory cells in its partial dependence on α4β7 integrins for lung recruitment.

Original languageEnglish (US)
Pages (from-to)1588-1594
Number of pages7
JournalBlood
Volume108
Issue number5
DOIs
StatePublished - Sep 1 2006

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Integrin alpha Chains
Vascular Cell Adhesion Molecule-1
Mast Cells
Integrins
Lung
Pneumonia
Monoclonal Antibodies
T-cells
Ovalbumin
Computer aided manufacturing
Blocking Antibodies
Adhesion
Inbred C57BL Mouse
Antigens
T-Lymphocytes

ASJC Scopus subject areas

  • Biochemistry
  • Immunology
  • Hematology
  • Cell Biology

Cite this

Abonia, J. P., Hallgren, J., Jones, T., Shi, T., Xu, Y., Koni, P., ... Gurish, M. F. (2006). Alpha-4 integrins and VCAM-1, but not MAdCAM-1, are essential for recruitment of mast cell progenitors to the inflamed lung. Blood, 108(5), 1588-1594. https://doi.org/10.1182/blood-2005-12-012781

Alpha-4 integrins and VCAM-1, but not MAdCAM-1, are essential for recruitment of mast cell progenitors to the inflamed lung. / Abonia, J. Pablo; Hallgren, Jenny; Jones, Tatiana; Shi, Tong; Xu, Yuhui; Koni, Pandelakis; Flavell, Richard A.; Boyce, Joshua A.; Austen, K. Frank; Gurish, Michael F.

In: Blood, Vol. 108, No. 5, 01.09.2006, p. 1588-1594.

Research output: Contribution to journalArticle

Abonia, JP, Hallgren, J, Jones, T, Shi, T, Xu, Y, Koni, P, Flavell, RA, Boyce, JA, Austen, KF & Gurish, MF 2006, 'Alpha-4 integrins and VCAM-1, but not MAdCAM-1, are essential for recruitment of mast cell progenitors to the inflamed lung', Blood, vol. 108, no. 5, pp. 1588-1594. https://doi.org/10.1182/blood-2005-12-012781
Abonia, J. Pablo ; Hallgren, Jenny ; Jones, Tatiana ; Shi, Tong ; Xu, Yuhui ; Koni, Pandelakis ; Flavell, Richard A. ; Boyce, Joshua A. ; Austen, K. Frank ; Gurish, Michael F. / Alpha-4 integrins and VCAM-1, but not MAdCAM-1, are essential for recruitment of mast cell progenitors to the inflamed lung. In: Blood. 2006 ; Vol. 108, No. 5. pp. 1588-1594.
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abstract = "Normal mouse lungs lack appreciable numbers of mast cells (MCs) or MC progenitors (MCp's), yet the appearance of mature MCs in the tracheobronchial epithelial surface is a characteristic of allergic, T-cell-dependent pulmonary inflammation. We hypothesized that pulmonary inflammation would recruit MCp's to inflamed lungs and that this recruitment would be regulated by distinct adhesion pathways. Ovalbumin-sensitized and challenged mice had a greater than 28-fold increase in the number of MCp's in the lungs. In mice lacking endothelial vascular cell adhesion molecule 1 (VCAM-1) and in wild-type mice administered blocking monoclonal antibody (mAb) to VCAM-1 but not to mucosal addressin CAM-1 (MadCAM-1), recruitment of MCp's to the inflamed lung was reduced by greater than 75{\%}. Analysis of the integrin receptors for VCAM-1 showed that in β7 integrin-deficient mice, recruitment was reduced 73{\%} relative to wild-type controls, and in either BALB/c or C57BL/6 mice, mAb blocking of α4, β1, or β7 integrins inhibited the recruitment of MCp's to the inflamed lung. Thus, VCAM-1 interactions with both α4β1 and α4β7 integrins are essential for the recruitment and expansion of the MCp populations in the lung during antigen-induced pulmonary inflammation. Furthermore, the MCp is currently unique among inflammatory cells in its partial dependence on α4β7 integrins for lung recruitment.",
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