Alteration of DMBA-induced oxidative stress by additive action of a modified indigenous preparation-Kalpaamruthaa

Arul Kumaran Shanmugam, Vanu Ramkumar Ramprasath, Palanivelu Shanthi, Panchanatham Sachdanandam

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

The present study investigated the protective efficacy of the novel preparation named as Kalpaamruthaa (KA, includes Semecarpus anacardium Linn nut milk extract (SA), dried powder of Phyllanthus emblica fruit and honey) on the peroxidative damage and abnormal antioxidant levels in the hepatic mitochondrial fraction of 7,12-dimethylbenz(a)anthracene (DMBA)-induced mammary carcinoma rats. Female Sprague-Dawley rats of weight 180 ± 10 g were categorized into six groups. Three groups were administered DMBA (25 mg/rat dissolved in olive oil, orally) to induce mammary carcinoma. One of these groups received KA treatment (300 mg/kg b.wt., orally) and other group received SA (200 mg/kg b.wt., orally) for 14 days after 90 days of DMBA induction. Vehicle-treated control and drug control groups were also included. The hepatic mitochondrial fraction of untreated DMBA rats showed 2.96-fold increase in MDA content when compared to control rats and abnormal changes in the activities/levels of mitochondrial enzymic (superoxide dismutase, glutathione peroxidase and glutathione reductase) and non-enzymic (glutathione, vitamin C and vitamin E) antioxidants were observed. DMBA-treated rats also showed decline in the activities of mitochondrial enzymes such as succinate dehydrogenase, α-ketoglutarate dehydrogenase, malate dehydrogenase and isocitrate dehydrogenase. In contrast, rats treated with SA and KA showed normal lipid peroxidation antioxidant defenses and mitochondrial enzymes, thereby showing the protection rendered by SA and KA. Although, KA treatment exhibited more profound effect in inhibiting DMBA-induced oxidative stress than sole SA treatment. Results of the study indicate that the anticarcinogenic activity of KA during DMBA-initiated mammary carcinogenesis is mediated through alteration of hepatic antioxidant status as well as modulation of TCA cycle enzymes. On the basis of the observed results, KA can be considered as a readily accessible, promising and novel cancer chemopreventive agent.

Original languageEnglish (US)
Pages (from-to)99-106
Number of pages8
JournalChemico-Biological Interactions
Volume167
Issue number2
DOIs
StatePublished - Apr 25 2007

Fingerprint

9,10-Dimethyl-1,2-benzanthracene
Oxidative stress
Oxidative Stress
Rats
Antioxidants
Semecarpus
Liver
Enzymes
Phyllanthus emblica
Rat control
Breast Neoplasms
Isocitrate Dehydrogenase
Malate Dehydrogenase
Nuts
Succinate Dehydrogenase
Honey
Glutathione Reductase
Drug and Narcotic Control
Glutathione Peroxidase
Fruits

Keywords

  • 7,12-Dimethylbenz(a)anthracene
  • Kalpaamruthaa
  • Mitochondria
  • Phyllanthus emblica
  • Reactive oxygen species
  • Semecarpus anacardium

ASJC Scopus subject areas

  • Toxicology

Cite this

Alteration of DMBA-induced oxidative stress by additive action of a modified indigenous preparation-Kalpaamruthaa. / Shanmugam, Arul Kumaran; Ramprasath, Vanu Ramkumar; Shanthi, Palanivelu; Sachdanandam, Panchanatham.

In: Chemico-Biological Interactions, Vol. 167, No. 2, 25.04.2007, p. 99-106.

Research output: Contribution to journalArticle

Shanmugam, Arul Kumaran ; Ramprasath, Vanu Ramkumar ; Shanthi, Palanivelu ; Sachdanandam, Panchanatham. / Alteration of DMBA-induced oxidative stress by additive action of a modified indigenous preparation-Kalpaamruthaa. In: Chemico-Biological Interactions. 2007 ; Vol. 167, No. 2. pp. 99-106.
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