Alterations in collagen subtype III and IV protein in experimental venous bypass grafting

Gregory J. Fulton, Keith M. Channon, Mark G. Davies, Brian H. Annex, Per Otto Hagen

Research output: Contribution to journalArticle

Abstract

Objective. To examine changes in collagen III and IV protein during the development of intimal hyperplasia in experimental vein grafts. Methods. Sixteen New Zealand White rabbits underwent reversed, jugular vein, interposition grafting of the carotid artery. Vessels were harvested 3, 7 or 28 days after operation and subjected to immunohistochemical examination and gelatinase assays. Results. In control vein, collagen IV was expressed around adventitial blood vessels and throughout the endothelium. Compared with its presence in control veins, collagen IV protein was decreased in the endothelium in all 3-day vein grafts and undetectable in the endothelium and intima in 7-day vein grafts, but was present in the endothelium and intimal hyperplasia in 28-day vein grafts. In contrast, collagen III was absent from the endothelium of control vein and 3-day vein grafts, was present at low levels in the intima of 7-day vein grafts, but was absent from the endothelium and intimal hyperplasia in 28-day vein grafts. In 3-day vein grafts, areas of collagen IV loss colocalized to areas of leukocyte infiltration. Protein extracts from 3-day vein grafts contained a 72 kDa gelatinase. Conclusions. The presence and alterations of collagen protein in veins and vein grafts are subtype specific. Collagen III does not appear to be a normal component of intimal hyperplasia in vein grafts. The decrease in collagen IV protein in the endothelium of veins may be a component of the endothelial changes that follow bypass grafting, mediated by leukocytes, the induction of gelatinase activity, or both.

Original languageEnglish (US)
Pages (from-to)191-197
Number of pages7
JournalCoronary Artery Disease
Volume9
Issue number4
StatePublished - Jun 29 1998
Externally publishedYes

Fingerprint

Veins
Collagen
Transplants
Proteins
Endothelium
Tunica Intima
Hyperplasia
Gelatinases
Leukocytes
Adventitia
Matrix Metalloproteinase 2
Jugular Veins
Carotid Arteries
Blood Vessels
Rabbits

Keywords

  • Endothelium
  • Intimal hyperplasia
  • Polymorphonuclear leukocytes
  • Vascular remodeling

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

Cite this

Fulton, G. J., Channon, K. M., Davies, M. G., Annex, B. H., & Hagen, P. O. (1998). Alterations in collagen subtype III and IV protein in experimental venous bypass grafting. Coronary Artery Disease, 9(4), 191-197.

Alterations in collagen subtype III and IV protein in experimental venous bypass grafting. / Fulton, Gregory J.; Channon, Keith M.; Davies, Mark G.; Annex, Brian H.; Hagen, Per Otto.

In: Coronary Artery Disease, Vol. 9, No. 4, 29.06.1998, p. 191-197.

Research output: Contribution to journalArticle

Fulton, GJ, Channon, KM, Davies, MG, Annex, BH & Hagen, PO 1998, 'Alterations in collagen subtype III and IV protein in experimental venous bypass grafting', Coronary Artery Disease, vol. 9, no. 4, pp. 191-197.
Fulton GJ, Channon KM, Davies MG, Annex BH, Hagen PO. Alterations in collagen subtype III and IV protein in experimental venous bypass grafting. Coronary Artery Disease. 1998 Jun 29;9(4):191-197.
Fulton, Gregory J. ; Channon, Keith M. ; Davies, Mark G. ; Annex, Brian H. ; Hagen, Per Otto. / Alterations in collagen subtype III and IV protein in experimental venous bypass grafting. In: Coronary Artery Disease. 1998 ; Vol. 9, No. 4. pp. 191-197.
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abstract = "Objective. To examine changes in collagen III and IV protein during the development of intimal hyperplasia in experimental vein grafts. Methods. Sixteen New Zealand White rabbits underwent reversed, jugular vein, interposition grafting of the carotid artery. Vessels were harvested 3, 7 or 28 days after operation and subjected to immunohistochemical examination and gelatinase assays. Results. In control vein, collagen IV was expressed around adventitial blood vessels and throughout the endothelium. Compared with its presence in control veins, collagen IV protein was decreased in the endothelium in all 3-day vein grafts and undetectable in the endothelium and intima in 7-day vein grafts, but was present in the endothelium and intimal hyperplasia in 28-day vein grafts. In contrast, collagen III was absent from the endothelium of control vein and 3-day vein grafts, was present at low levels in the intima of 7-day vein grafts, but was absent from the endothelium and intimal hyperplasia in 28-day vein grafts. In 3-day vein grafts, areas of collagen IV loss colocalized to areas of leukocyte infiltration. Protein extracts from 3-day vein grafts contained a 72 kDa gelatinase. Conclusions. The presence and alterations of collagen protein in veins and vein grafts are subtype specific. Collagen III does not appear to be a normal component of intimal hyperplasia in vein grafts. The decrease in collagen IV protein in the endothelium of veins may be a component of the endothelial changes that follow bypass grafting, mediated by leukocytes, the induction of gelatinase activity, or both.",
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AU - Hagen, Per Otto

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N2 - Objective. To examine changes in collagen III and IV protein during the development of intimal hyperplasia in experimental vein grafts. Methods. Sixteen New Zealand White rabbits underwent reversed, jugular vein, interposition grafting of the carotid artery. Vessels were harvested 3, 7 or 28 days after operation and subjected to immunohistochemical examination and gelatinase assays. Results. In control vein, collagen IV was expressed around adventitial blood vessels and throughout the endothelium. Compared with its presence in control veins, collagen IV protein was decreased in the endothelium in all 3-day vein grafts and undetectable in the endothelium and intima in 7-day vein grafts, but was present in the endothelium and intimal hyperplasia in 28-day vein grafts. In contrast, collagen III was absent from the endothelium of control vein and 3-day vein grafts, was present at low levels in the intima of 7-day vein grafts, but was absent from the endothelium and intimal hyperplasia in 28-day vein grafts. In 3-day vein grafts, areas of collagen IV loss colocalized to areas of leukocyte infiltration. Protein extracts from 3-day vein grafts contained a 72 kDa gelatinase. Conclusions. The presence and alterations of collagen protein in veins and vein grafts are subtype specific. Collagen III does not appear to be a normal component of intimal hyperplasia in vein grafts. The decrease in collagen IV protein in the endothelium of veins may be a component of the endothelial changes that follow bypass grafting, mediated by leukocytes, the induction of gelatinase activity, or both.

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