Altered regulation of heat shock gene expression in heat resistant mouse cells

Young Mee K. Park, Nahid F Mivechi, Elizabeth A. Auger, George M. Hahn

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Purpose: The differences in the heat shock gene regulation between the RIF-1 cell line and its heat resistant derivative TR4 are further characterized. Methods and Materials: In vitro gel retardation assays were used to assess the presence of activated heat shock transcription factor in the two cell lines. The levels of the heat-inducible HSP 70.1, the constitutive HSC 70, the germ line-specific HSP 70.2, and the HSP 28 mRNAs in both untreated and iso-heated RIF-1 and TR4 cells were determined using the polymerase chain reaction coupled with the reverse transcriptase reaction. Induction and decay of induced heat shock protein synthesis was measured by 35S-methionine labeling of proteins. Results: Unheated TR4 cells display characteristics of heat shocked RIF-1 cells. TR4 cells have a constitutively activated heat shock transcription factor and elevated levels of the HSP 70.1, HSC 70, and the HSP 28 mRNAs. Upon an equal heat dose of 45 C, 15 min, the TR4 cells exhibited a more rapid onset in heat shock mRNA and protein induction than did the RIF-1 cells. During the recovery from heat shock, the activated heat shock transcription factor and the induced HSP70 mRNAs decayed more slowly in the TR4 cells, although the protein synthesis pattern of the TR4 cells returned to control levels more rapidly following heat shock than did protein synthesis of the RIF-1 cells. Conclusion: Unheated TR4 cells are similar to heat shocked RIF-1 cells at the transcriptional level. Induced HSP70 expression is modulated by the severity of the heat treatment (or the degree of heat damage) perceived by the cells rather than by the absolute heat dose given. We propose that the unheated TR4 cells are locked into the "ON" state of the heat shock response.

Original languageEnglish (US)
Pages (from-to)179-187
Number of pages9
JournalInternational Journal of Radiation Oncology, Biology, Physics
Volume28
Issue number1
DOIs
StatePublished - Jan 1 1994
Externally publishedYes

Fingerprint

gene expression
mice
Shock
Hot Temperature
shock
Gene Expression
heat
cells
protein synthesis
Heat-Shock Proteins
Messenger RNA
cultured cells
induction
Heat-Shock Response
Cell Line
RNA-Directed DNA Polymerase
proteins
Electrophoretic Mobility Shift Assay
polymerase chain reaction
methionine

Keywords

  • HSC 70
  • HSP 28
  • HSP 70.1
  • HSP 70.2
  • Heat resistance
  • Heat shock proteins
  • Heat shock transcription factor
  • Polymerase chain reaction

ASJC Scopus subject areas

  • Radiation
  • Oncology
  • Radiology Nuclear Medicine and imaging
  • Cancer Research

Cite this

Altered regulation of heat shock gene expression in heat resistant mouse cells. / Park, Young Mee K.; Mivechi, Nahid F; Auger, Elizabeth A.; Hahn, George M.

In: International Journal of Radiation Oncology, Biology, Physics, Vol. 28, No. 1, 01.01.1994, p. 179-187.

Research output: Contribution to journalArticle

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abstract = "Purpose: The differences in the heat shock gene regulation between the RIF-1 cell line and its heat resistant derivative TR4 are further characterized. Methods and Materials: In vitro gel retardation assays were used to assess the presence of activated heat shock transcription factor in the two cell lines. The levels of the heat-inducible HSP 70.1, the constitutive HSC 70, the germ line-specific HSP 70.2, and the HSP 28 mRNAs in both untreated and iso-heated RIF-1 and TR4 cells were determined using the polymerase chain reaction coupled with the reverse transcriptase reaction. Induction and decay of induced heat shock protein synthesis was measured by 35S-methionine labeling of proteins. Results: Unheated TR4 cells display characteristics of heat shocked RIF-1 cells. TR4 cells have a constitutively activated heat shock transcription factor and elevated levels of the HSP 70.1, HSC 70, and the HSP 28 mRNAs. Upon an equal heat dose of 45 C, 15 min, the TR4 cells exhibited a more rapid onset in heat shock mRNA and protein induction than did the RIF-1 cells. During the recovery from heat shock, the activated heat shock transcription factor and the induced HSP70 mRNAs decayed more slowly in the TR4 cells, although the protein synthesis pattern of the TR4 cells returned to control levels more rapidly following heat shock than did protein synthesis of the RIF-1 cells. Conclusion: Unheated TR4 cells are similar to heat shocked RIF-1 cells at the transcriptional level. Induced HSP70 expression is modulated by the severity of the heat treatment (or the degree of heat damage) perceived by the cells rather than by the absolute heat dose given. We propose that the unheated TR4 cells are locked into the {"}ON{"} state of the heat shock response.",
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