Alternative splicing and hypermutation of a nonproductively rearranged TCR α-chain in a T cell hybridoma

Brendan Marshall, Ruth Schulz, Min Zhou, Andrew Mellor

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Like Ig genes, TCR genes are formed by somatic rearrangements of noncontiguous genomic V, J, and C regions. Unlike Ig genes, somatic hypermutation of TCR V regions is an infrequent event. We describe the occurrence of spontaneous hypermutation in a nonproductively rearranged TCR α-chain gene in a clonal T cell hybridoma that had lost its productively rearranged α-chain. The mutating hybridoma was eventually supplanted in culture by a nonmutating variant that had restored an open reading frame in the nonproductively rearranged TCR α-chain through the use of cryptic splice sites in the Vα region. Evidence is presented for the presence of cDNA reverse transcripts of the TCR α-chain within the hybridoma, suggesting a role for reverse transcriptase in the generation of mutations.

Original languageEnglish (US)
Pages (from-to)871-877
Number of pages7
JournalJournal of Immunology
Volume162
Issue number2
StatePublished - Jan 15 1999

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Hybridomas
Alternative Splicing
Immunoglobulin Genes
T-Lymphocytes
Immunoglobulin Somatic Hypermutation
RNA Splice Sites
RNA-Directed DNA Polymerase
Open Reading Frames
Genes
Complementary DNA
Mutation

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Alternative splicing and hypermutation of a nonproductively rearranged TCR α-chain in a T cell hybridoma. / Marshall, Brendan; Schulz, Ruth; Zhou, Min; Mellor, Andrew.

In: Journal of Immunology, Vol. 162, No. 2, 15.01.1999, p. 871-877.

Research output: Contribution to journalArticle

Marshall, B, Schulz, R, Zhou, M & Mellor, A 1999, 'Alternative splicing and hypermutation of a nonproductively rearranged TCR α-chain in a T cell hybridoma', Journal of Immunology, vol. 162, no. 2, pp. 871-877.
Marshall, Brendan ; Schulz, Ruth ; Zhou, Min ; Mellor, Andrew. / Alternative splicing and hypermutation of a nonproductively rearranged TCR α-chain in a T cell hybridoma. In: Journal of Immunology. 1999 ; Vol. 162, No. 2. pp. 871-877.
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