Amplification of JC virus DNA from brain and cerebrospinal fluid of patients with progressive multifocal leukoencephalopathy

John Henson, Marc Rosenblum, Donald Armstrong, Henry Furneaux

Research output: Contribution to journalArticlepeer-review

58 Scopus citations

Abstract

We have established an assay to detect JC viral DNA in brain tissue and CSF of patients with progressive multifocal leukoencephalopathy (PML) and amplified a 106-base pair segment of the gene encoding JC viral large T antigen by polymerase chain reaction (PCR) from the brains of eight patients with PML and from one of six CSF samples. Brain and C SF of patients without PML did not yield JC virus-specific DNA amplification products. Southern analysis documented that the sequences of the 106-base pair PCR product and JC viral DNA are identical. This assay can be used in the diagnosis of PML and to investigate the biology of JC virus.

Original languageEnglish (US)
Pages (from-to)1967-1971
Number of pages5
JournalNeurology
Volume41
Issue number12
DOIs
StatePublished - Dec 1991
Externally publishedYes

ASJC Scopus subject areas

  • Clinical Neurology

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