Abstract
We have established an assay to detect JC viral DNA in brain tissue and CSF of patients with progressive multifocal leukoencephalopathy (PML) and amplified a 106-base pair segment of the gene encoding JC viral large T antigen by polymerase chain reaction (PCR) from the brains of eight patients with PML and from one of six CSF samples. Brain and C SF of patients without PML did not yield JC virus-specific DNA amplification products. Southern analysis documented that the sequences of the 106-base pair PCR product and JC viral DNA are identical. This assay can be used in the diagnosis of PML and to investigate the biology of JC virus.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1967-1971 |
| Number of pages | 5 |
| Journal | Neurology |
| Volume | 41 |
| Issue number | 12 |
| DOIs | |
| State | Published - Dec 1991 |
| Externally published | Yes |
ASJC Scopus subject areas
- Clinical Neurology