An Analysis of γ‐Aminobutyrate Receptors and Uptake by Isolated Purkinje Cells

Fritz A. Henn, Richard C Venema, David Anderson, Ake Sellstrom

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Abstract: An isolated fraction of Purkinje perikarya was prepared. This fraction had [3H]GABA receptor binding and [3H]muscimol binding analogous to that reported for heterogeneous cerebellar membranes. The finding of two binding components with each ligand suggests that these components do not represent two binding sites, one presynaptic and the other postsynaptic, since both are clearly seen on purified Purkinje cell bodies. Subcellular fractionation indicates that synaptic endings and plasma membranes are enriched in GABA receptors compared with intracellular organelles. Purkinje cell bodies were also found to possess a high‐affinity transport system for GABA, which was sensitive to inhibition by diaminobutyric acid (DABA) but not by β‐alanine. They showed no evidence of homoexchange (the movement of label without net transport). This supports our suggestion that homoexchange is an artifact of synaptic particle formation.

Original languageEnglish (US)
Pages (from-to)1671-1677
Number of pages7
JournalJournal of Neurochemistry
Volume34
Issue number6
DOIs
StatePublished - Jan 1 1980

Fingerprint

Aminobutyrates
GABA Receptors
Purkinje Cells
Cells
Synaptic Membranes
Muscimol
Cell membranes
Fractionation
Alanine
Organelles
Artifacts
gamma-Aminobutyric Acid
Labels
Binding Sites
Cell Membrane
Ligands
Membranes
Acids
Cell Body

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

Cite this

An Analysis of γ‐Aminobutyrate Receptors and Uptake by Isolated Purkinje Cells. / Henn, Fritz A.; Venema, Richard C; Anderson, David; Sellstrom, Ake.

In: Journal of Neurochemistry, Vol. 34, No. 6, 01.01.1980, p. 1671-1677.

Research output: Contribution to journalArticle

Henn, Fritz A. ; Venema, Richard C ; Anderson, David ; Sellstrom, Ake. / An Analysis of γ‐Aminobutyrate Receptors and Uptake by Isolated Purkinje Cells. In: Journal of Neurochemistry. 1980 ; Vol. 34, No. 6. pp. 1671-1677.
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