An artery-specific fluorescent dye for studying neurovascular coupling

Zhiming Shen, Zhongyang Lu, Pratik Y. Chhatbar, Philip O'Herron, Prakash Kara

Research output: Contribution to journalArticle

67 Citations (Scopus)

Abstract

We demonstrate that Alexa Fluor 633 hydrazide (Alexa Fluor 633) selectively labels neocortical arteries and arterioles by binding to elastin fibers. We measured sensory stimulus-evoked arteriole dilation dynamics in mouse, rat and cat visual cortex using Alexa Fluor 633 together with neuronal activity using calcium indicators or blood flow using fluorescein dextran. Arteriole dilation decreased fluorescence recorded from immediately underlying neurons, representing a potential artifact during neuronal functional imaging experiments.

Original languageEnglish (US)
Pages (from-to)273-276
Number of pages4
JournalNature Methods
Volume9
Issue number3
DOIs
StatePublished - Mar 1 2012

Fingerprint

Elastin
Arterioles
Fluorescent Dyes
Neurons
Rats
Labels
Blood
Arteries
Fluorescence
Calcium
Imaging techniques
Fibers
Dilatation
Experiments
Visual Cortex
Artifacts
Cats
fluorescein-dextran
Neurovascular Coupling

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

An artery-specific fluorescent dye for studying neurovascular coupling. / Shen, Zhiming; Lu, Zhongyang; Chhatbar, Pratik Y.; O'Herron, Philip; Kara, Prakash.

In: Nature Methods, Vol. 9, No. 3, 01.03.2012, p. 273-276.

Research output: Contribution to journalArticle

Shen, Zhiming ; Lu, Zhongyang ; Chhatbar, Pratik Y. ; O'Herron, Philip ; Kara, Prakash. / An artery-specific fluorescent dye for studying neurovascular coupling. In: Nature Methods. 2012 ; Vol. 9, No. 3. pp. 273-276.
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