An autoregulatory enhancer element of the Drosophila homeotic gene Deformed

Clare M Bergson, W. McGinnis

Research output: Contribution to journalArticle

64 Citations (Scopus)

Abstract

The stable determination of different anterior-posterior regions of the Drosophila embryo is controlled by the persistent expression of homeotic selector genes. One mechanism that has been proposed to explain the persistent expression of the homeotic gene Deformed is an autoactivation circuit that would be used once Deformed expression had been established by earlier acting patterning genes. Here we show that a large cis-regulatory element mapping ~5 kb upstream of the Deformed transcription start has the properties predicted for a Deformed autoregulatory enhancer. This element provides late, spatially localized expression in the epidermal cells of the maxillary and mandibular segments which is wholly dependent upon endogenous Deformed function. In addition, the autoregulatory enhancer can be activated ectopically in embryos and in imaginal disc cells by ectopic expression of Deformed protein. Deletion analysis of the autoregulatory element indicates that it contains compartment specific sub-elements similar to those of other homeotic loci.

Original languageEnglish (US)
Pages (from-to)4287-4297
Number of pages11
JournalEMBO Journal
Volume9
Issue number13
StatePublished - Dec 1 1990
Externally publishedYes

Fingerprint

Homeobox Genes
Drosophila
Embryonic Structures
Genes
Imaginal Discs
Transcription
Proteins
Networks (circuits)
Ectopic Gene Expression

Keywords

  • autoregulation
  • Deformed
  • determination
  • enhancer
  • homeotic

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

Cite this

An autoregulatory enhancer element of the Drosophila homeotic gene Deformed. / Bergson, Clare M; McGinnis, W.

In: EMBO Journal, Vol. 9, No. 13, 01.12.1990, p. 4287-4297.

Research output: Contribution to journalArticle

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