Abstract
The localization of ASH1 mRNA to the distal tip of budding yeast cells is essential for the proper regulation of mating type switching in Saccharomyces cerevisiae. A localization element that is predominantly in the 3′-untranslated region (UTR) can direct this mRNA to the bud. Using this element in the three-hybrid in vivo RNA-binding assay, we identified a protein, Loc1p, that binds in vitro directly to the wild-type ASH1 3′-UTR RNA, but not to a mutant RNA incapable of localizing to the bud nor to several other mRNAs. LOC1 codes for a novel protein that recognizes double-stranded RNA structures and is required for efficient localization of ASH1 mRNA. Accordingly, Ash1p gets symmetrically distributed between daughter and mother cells in a locl strain. Surprisingly, Loc1p was found to be strictly nuclear, unlike other known RNA-binding proteins involved in mRNA localization which shuttle between the nucleus and the cytoplasm. We propose that efficient cytoplasmic ASH1 mRNA localization requires a previous interaction with specific nuclear factors.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 307-318 |
| Number of pages | 12 |
| Journal | Journal of Cell Biology |
| Volume | 153 |
| Issue number | 2 |
| DOIs | |
| State | Published - Apr 16 2001 |
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Keywords
- ASH1
- Nuclear RNA-binding protein
- RNA localization
- Three-hybrid
- Yeast
ASJC Scopus subject areas
- Cell Biology
Cite this
An exclusively nuclear RNA-binding protein affects asymmetric localization of ASH1 mRNA and Ash1p in yeast. / Long, Roy M.; Gu, Wei; Meng, Xiuhua; Gonsalvez, Graydon; Singer, Robert H.; Chartrand, Pascal.
In: Journal of Cell Biology, Vol. 153, No. 2, 16.04.2001, p. 307-318.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - An exclusively nuclear RNA-binding protein affects asymmetric localization of ASH1 mRNA and Ash1p in yeast
AU - Long, Roy M.
AU - Gu, Wei
AU - Meng, Xiuhua
AU - Gonsalvez, Graydon
AU - Singer, Robert H.
AU - Chartrand, Pascal
PY - 2001/4/16
Y1 - 2001/4/16
N2 - The localization of ASH1 mRNA to the distal tip of budding yeast cells is essential for the proper regulation of mating type switching in Saccharomyces cerevisiae. A localization element that is predominantly in the 3′-untranslated region (UTR) can direct this mRNA to the bud. Using this element in the three-hybrid in vivo RNA-binding assay, we identified a protein, Loc1p, that binds in vitro directly to the wild-type ASH1 3′-UTR RNA, but not to a mutant RNA incapable of localizing to the bud nor to several other mRNAs. LOC1 codes for a novel protein that recognizes double-stranded RNA structures and is required for efficient localization of ASH1 mRNA. Accordingly, Ash1p gets symmetrically distributed between daughter and mother cells in a locl strain. Surprisingly, Loc1p was found to be strictly nuclear, unlike other known RNA-binding proteins involved in mRNA localization which shuttle between the nucleus and the cytoplasm. We propose that efficient cytoplasmic ASH1 mRNA localization requires a previous interaction with specific nuclear factors.
AB - The localization of ASH1 mRNA to the distal tip of budding yeast cells is essential for the proper regulation of mating type switching in Saccharomyces cerevisiae. A localization element that is predominantly in the 3′-untranslated region (UTR) can direct this mRNA to the bud. Using this element in the three-hybrid in vivo RNA-binding assay, we identified a protein, Loc1p, that binds in vitro directly to the wild-type ASH1 3′-UTR RNA, but not to a mutant RNA incapable of localizing to the bud nor to several other mRNAs. LOC1 codes for a novel protein that recognizes double-stranded RNA structures and is required for efficient localization of ASH1 mRNA. Accordingly, Ash1p gets symmetrically distributed between daughter and mother cells in a locl strain. Surprisingly, Loc1p was found to be strictly nuclear, unlike other known RNA-binding proteins involved in mRNA localization which shuttle between the nucleus and the cytoplasm. We propose that efficient cytoplasmic ASH1 mRNA localization requires a previous interaction with specific nuclear factors.
KW - ASH1
KW - Nuclear RNA-binding protein
KW - RNA localization
KW - Three-hybrid
KW - Yeast
UR - http://www.scopus.com/inward/record.url?scp=0035897422&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0035897422&partnerID=8YFLogxK
U2 - 10.1083/jcb.153.2.307
DO - 10.1083/jcb.153.2.307
M3 - Article
C2 - 11309412
AN - SCOPUS:0035897422
VL - 153
SP - 307
EP - 318
JO - Journal of Cell Biology
JF - Journal of Cell Biology
SN - 0021-9525
IS - 2
ER -