An exclusively nuclear RNA-binding protein affects asymmetric localization of ASH1 mRNA and Ash1p in yeast

Roy M. Long, Wei Gu, Xiuhua Meng, Graydon Gonsalvez, Robert H. Singer, Pascal Chartrand

Research output: Contribution to journalArticle

73 Citations (Scopus)

Abstract

The localization of ASH1 mRNA to the distal tip of budding yeast cells is essential for the proper regulation of mating type switching in Saccharomyces cerevisiae. A localization element that is predominantly in the 3′-untranslated region (UTR) can direct this mRNA to the bud. Using this element in the three-hybrid in vivo RNA-binding assay, we identified a protein, Loc1p, that binds in vitro directly to the wild-type ASH1 3′-UTR RNA, but not to a mutant RNA incapable of localizing to the bud nor to several other mRNAs. LOC1 codes for a novel protein that recognizes double-stranded RNA structures and is required for efficient localization of ASH1 mRNA. Accordingly, Ash1p gets symmetrically distributed between daughter and mother cells in a locl strain. Surprisingly, Loc1p was found to be strictly nuclear, unlike other known RNA-binding proteins involved in mRNA localization which shuttle between the nucleus and the cytoplasm. We propose that efficient cytoplasmic ASH1 mRNA localization requires a previous interaction with specific nuclear factors.

Original languageEnglish (US)
Pages (from-to)307-318
Number of pages12
JournalJournal of Cell Biology
Volume153
Issue number2
DOIs
StatePublished - Apr 16 2001

Fingerprint

Nuclear RNA
RNA-Binding Proteins
Nuclear Proteins
Yeasts
Messenger RNA
3' Untranslated Regions
RNA
Untranslated RNA
Saccharomycetales
Double-Stranded RNA
Saccharomyces cerevisiae
Cytoplasm
Proteins
Stem Cells

Keywords

  • ASH1
  • Nuclear RNA-binding protein
  • RNA localization
  • Three-hybrid
  • Yeast

ASJC Scopus subject areas

  • Cell Biology

Cite this

An exclusively nuclear RNA-binding protein affects asymmetric localization of ASH1 mRNA and Ash1p in yeast. / Long, Roy M.; Gu, Wei; Meng, Xiuhua; Gonsalvez, Graydon; Singer, Robert H.; Chartrand, Pascal.

In: Journal of Cell Biology, Vol. 153, No. 2, 16.04.2001, p. 307-318.

Research output: Contribution to journalArticle

Long, Roy M. ; Gu, Wei ; Meng, Xiuhua ; Gonsalvez, Graydon ; Singer, Robert H. ; Chartrand, Pascal. / An exclusively nuclear RNA-binding protein affects asymmetric localization of ASH1 mRNA and Ash1p in yeast. In: Journal of Cell Biology. 2001 ; Vol. 153, No. 2. pp. 307-318.
@article{f24c5cd396c14b10b9aa10fd7d5b78e2,
title = "An exclusively nuclear RNA-binding protein affects asymmetric localization of ASH1 mRNA and Ash1p in yeast",
abstract = "The localization of ASH1 mRNA to the distal tip of budding yeast cells is essential for the proper regulation of mating type switching in Saccharomyces cerevisiae. A localization element that is predominantly in the 3′-untranslated region (UTR) can direct this mRNA to the bud. Using this element in the three-hybrid in vivo RNA-binding assay, we identified a protein, Loc1p, that binds in vitro directly to the wild-type ASH1 3′-UTR RNA, but not to a mutant RNA incapable of localizing to the bud nor to several other mRNAs. LOC1 codes for a novel protein that recognizes double-stranded RNA structures and is required for efficient localization of ASH1 mRNA. Accordingly, Ash1p gets symmetrically distributed between daughter and mother cells in a locl strain. Surprisingly, Loc1p was found to be strictly nuclear, unlike other known RNA-binding proteins involved in mRNA localization which shuttle between the nucleus and the cytoplasm. We propose that efficient cytoplasmic ASH1 mRNA localization requires a previous interaction with specific nuclear factors.",
keywords = "ASH1, Nuclear RNA-binding protein, RNA localization, Three-hybrid, Yeast",
author = "Long, {Roy M.} and Wei Gu and Xiuhua Meng and Graydon Gonsalvez and Singer, {Robert H.} and Pascal Chartrand",
year = "2001",
month = "4",
day = "16",
doi = "10.1083/jcb.153.2.307",
language = "English (US)",
volume = "153",
pages = "307--318",
journal = "Journal of Cell Biology",
issn = "0021-9525",
publisher = "Rockefeller University Press",
number = "2",

}

TY - JOUR

T1 - An exclusively nuclear RNA-binding protein affects asymmetric localization of ASH1 mRNA and Ash1p in yeast

AU - Long, Roy M.

AU - Gu, Wei

AU - Meng, Xiuhua

AU - Gonsalvez, Graydon

AU - Singer, Robert H.

AU - Chartrand, Pascal

PY - 2001/4/16

Y1 - 2001/4/16

N2 - The localization of ASH1 mRNA to the distal tip of budding yeast cells is essential for the proper regulation of mating type switching in Saccharomyces cerevisiae. A localization element that is predominantly in the 3′-untranslated region (UTR) can direct this mRNA to the bud. Using this element in the three-hybrid in vivo RNA-binding assay, we identified a protein, Loc1p, that binds in vitro directly to the wild-type ASH1 3′-UTR RNA, but not to a mutant RNA incapable of localizing to the bud nor to several other mRNAs. LOC1 codes for a novel protein that recognizes double-stranded RNA structures and is required for efficient localization of ASH1 mRNA. Accordingly, Ash1p gets symmetrically distributed between daughter and mother cells in a locl strain. Surprisingly, Loc1p was found to be strictly nuclear, unlike other known RNA-binding proteins involved in mRNA localization which shuttle between the nucleus and the cytoplasm. We propose that efficient cytoplasmic ASH1 mRNA localization requires a previous interaction with specific nuclear factors.

AB - The localization of ASH1 mRNA to the distal tip of budding yeast cells is essential for the proper regulation of mating type switching in Saccharomyces cerevisiae. A localization element that is predominantly in the 3′-untranslated region (UTR) can direct this mRNA to the bud. Using this element in the three-hybrid in vivo RNA-binding assay, we identified a protein, Loc1p, that binds in vitro directly to the wild-type ASH1 3′-UTR RNA, but not to a mutant RNA incapable of localizing to the bud nor to several other mRNAs. LOC1 codes for a novel protein that recognizes double-stranded RNA structures and is required for efficient localization of ASH1 mRNA. Accordingly, Ash1p gets symmetrically distributed between daughter and mother cells in a locl strain. Surprisingly, Loc1p was found to be strictly nuclear, unlike other known RNA-binding proteins involved in mRNA localization which shuttle between the nucleus and the cytoplasm. We propose that efficient cytoplasmic ASH1 mRNA localization requires a previous interaction with specific nuclear factors.

KW - ASH1

KW - Nuclear RNA-binding protein

KW - RNA localization

KW - Three-hybrid

KW - Yeast

UR - http://www.scopus.com/inward/record.url?scp=0035897422&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0035897422&partnerID=8YFLogxK

U2 - 10.1083/jcb.153.2.307

DO - 10.1083/jcb.153.2.307

M3 - Article

C2 - 11309412

AN - SCOPUS:0035897422

VL - 153

SP - 307

EP - 318

JO - Journal of Cell Biology

JF - Journal of Cell Biology

SN - 0021-9525

IS - 2

ER -