An immunofluorescent analysis of lectin binding to normal and regenerating skeletal muscle of rat

Adarsh K Gulati, Andrew A. Zalewski

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Ten different fluorescein‐conjugated lectins of various known sugar specificities were used to study cell surface glycoconjugates of normal and regenerating rat skeletal muscle. In normal muscle, Canavalia ensiformis agglutinin, Triticum vulgaris agglutinin (wheat germ agglutinin, WGA), Ricinus communis agglutinin‐I, and Maclura pomifera agglutinin bound strongly to the endomysial region of the myofibers. No binding was observed in the cytoplasm of the myofibers. Other lectins (Dolichos biflorus agglutinin, Griffonia simplifolia agglutinin I and II, Ulex europaeus agglutinin, Arachis hypogaea agglutinin, Glycine max agglutinin) bound very poorly or not at all in the normal muscle. Skeletal muscle degeneration and regeneration was induced by autotransplantation of the extensor digitorum longus muscle. In the transplanted muscles, the endomysial lectin binding of the degenerating myofibers became weak and lacked continuity, indicating a breakdown of the endomysium. Of all the lectins tested, only WGA revealed intense binding in the myogenic zone of regenerating muscle. As the regeneration progressed, this WGA binding became restricted to the new endomysium. In completely regenerated muscle, binding of all lectins was similar to that seen in normal muscle. The specific binding of WGA to the myogenic zone may be important in identifying factors or glycoconjugates, which constitute a favorable environmnt for skeletal muscle regeneration.

Original languageEnglish (US)
Pages (from-to)113-117
Number of pages5
JournalThe Anatomical Record
Volume212
Issue number2
DOIs
StatePublished - Jan 1 1985

Fingerprint

agglutinins
Agglutinins
Lectins
lectins
skeletal muscle
Skeletal Muscle
muscle
Wheat Germ Agglutinins
Muscles
rats
wheat germ
Regeneration
muscles
Glycoconjugates
wheat
Maclura
regeneration
Canavalia
Griffonia
glycoconjugates

ASJC Scopus subject areas

  • Biotechnology
  • Anatomy
  • Ecology, Evolution, Behavior and Systematics
  • Histology

Cite this

An immunofluorescent analysis of lectin binding to normal and regenerating skeletal muscle of rat. / Gulati, Adarsh K; Zalewski, Andrew A.

In: The Anatomical Record, Vol. 212, No. 2, 01.01.1985, p. 113-117.

Research output: Contribution to journalArticle

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