An immunohistochemical study of collagen types III, VI and IX in rabbit craniomandibular joint tissues following surgical induction of anterior disk displacement

Ayman M. Ali, Mohamed M. Sharawy

Research output: Contribution to journalArticlepeer-review

22 Scopus citations

Abstract

The purpose of this study was to determine the effect of surgical induction of anterior disk displacement (ADD) on type-III, VI and IX collagens of the rabbit craniomandibular joint (CMJ) tissues using an immunohistochemical technique. The right joint was exposed surgically, all discal attachments were severed except for the posterior discal attachment (bilaminar zone). The disk was then repositioned anteriorly and sutured to the zygomatic arch. The left joint served as a sham-operated control. Ten additional joints were used as non-operated controls. Deeply anesthetized rabbits were perfused with 2% buffered formalin 2 weeks (10 rabbits) or 6 weeks (10 rabbits) following surgery. The articular disk, bilaminar zone, mandibular condyle and articular eminence were excised. The last two were decalcified in EDTA. All tissues were then sectioned at 10 μm in a cryostat. Sections were incubated with monoclonal antibodies directed against type-III, VI or IX collagens. Following incubation in the appropriate FITC-labelled secondary antibodies, all sections were studied under the fluorescence microscope. The results showed a reduction in immunostaining for type-VI and IX collagens in the condylar cartilage, disk and articular eminence at 2 weeks, followed by an increase in their immunostaining at 6 weeks and the appearance of a de novo type-III collagen in the condylar cartilage and the articular eminence. It is concluded that surgical induction of ADD in the rabbit CMJ leads to alterations in its type-III, VI and IX collagens.

Original languageEnglish (US)
Pages (from-to)78-85
Number of pages8
JournalJournal of Oral Pathology and Medicine
Volume25
Issue number2
DOIs
StatePublished - Jan 1 1996

Keywords

  • Anterior disk displacement
  • Craniomandibular joint
  • Minor types of collagen
  • Osteoarthritis
  • Temporomandibular joint

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Oral Surgery
  • Otorhinolaryngology
  • Cancer Research
  • Periodontics

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