An immunosuppressive factor interacts with membrane glycolipid asialo-GM1 structure

K. C. Wang, C. R. Verret, Robert K Yu, R. K. Gershon, S. Lee

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

An immunosuppressive factor was isolated from sheep-erythrocyte hyperimmunized mouseserum. Possibilities of the adsorption of the immunosuppressive factor with glycolipid asialo-GM1 incorporated liposome (dipalmitoyl lecithin) and the blockade of immunosuppressive activity by specific anti-asialo-GM1 incorporated antibody were tested. We report here that: 1) The immunosuppressive activity of the immunosuppressive factor decreased quantitatively if the factor was preincubated with asialo-GM1 incorporated liposome (GALP) as compared with that preincubated with liposome (LP) alone; and 2) the presence of specific anti-asialo GM1 antibody is able to protect in vitro anti-sheep erythrocyte (SRBC) response from the suppressive effect of the immunosuppressive factor in a dose-dependent fashion under similar cell culture condition. The results indicate that asialo-GM1 may be related to the receptor of immunosuppressive factor.

Original languageEnglish (US)
Pages (from-to)139-145
Number of pages7
JournalImmunobiology
Volume174
Issue number2
DOIs
StatePublished - Jan 1 1987

Fingerprint

Immunosuppressive Agents
Membranes
Liposomes
Sheep
Erythrocytes
Lecithins
Antibodies
asialo GM1 ganglioside
Adsorption
Cell Culture Techniques

Keywords

  • CSF
  • DPL
  • Ig
  • L-dipalmitoyl lecithin
  • MAF
  • MIF
  • PFC
  • SF
  • SRBC
  • colony stimulating factor
  • immunoglobulin
  • immunosuppressive factor
  • macrophage activating factor
  • macrophage migration inhibition factor
  • plaque forming cell
  • sheep erythrocyte

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Hematology

Cite this

An immunosuppressive factor interacts with membrane glycolipid asialo-GM1 structure. / Wang, K. C.; Verret, C. R.; Yu, Robert K; Gershon, R. K.; Lee, S.

In: Immunobiology, Vol. 174, No. 2, 01.01.1987, p. 139-145.

Research output: Contribution to journalArticle

Wang, K. C. ; Verret, C. R. ; Yu, Robert K ; Gershon, R. K. ; Lee, S. / An immunosuppressive factor interacts with membrane glycolipid asialo-GM1 structure. In: Immunobiology. 1987 ; Vol. 174, No. 2. pp. 139-145.
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