Abstract
An immunosuppressive factor was isolated from sheep-erythrocyte hyperimmunized mouseserum. Possibilities of the adsorption of the immunosuppressive factor with glycolipid asialo-GM1 incorporated liposome (dipalmitoyl lecithin) and the blockade of immunosuppressive activity by specific anti-asialo-GM1 incorporated antibody were tested. We report here that: 1) The immunosuppressive activity of the immunosuppressive factor decreased quantitatively if the factor was preincubated with asialo-GM1 incorporated liposome (GALP) as compared with that preincubated with liposome (LP) alone; and 2) the presence of specific anti-asialo GM1 antibody is able to protect in vitro anti-sheep erythrocyte (SRBC) response from the suppressive effect of the immunosuppressive factor in a dose-dependent fashion under similar cell culture condition. The results indicate that asialo-GM1 may be related to the receptor of immunosuppressive factor.
Original language | English (US) |
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Pages (from-to) | 139-145 |
Number of pages | 7 |
Journal | Immunobiology |
Volume | 174 |
Issue number | 2 |
DOIs | |
State | Published - 1987 |
Keywords
- CSF
- DPL
- Ig
- L-dipalmitoyl lecithin
- MAF
- MIF
- PFC
- SF
- SRBC
- colony stimulating factor
- immunoglobulin
- immunosuppressive factor
- macrophage activating factor
- macrophage migration inhibition factor
- plaque forming cell
- sheep erythrocyte
ASJC Scopus subject areas
- Immunology and Allergy
- Immunology
- Hematology