An in vitro evaluation of the cytotoxicity of various endodontic irrigants on human gingival fibroblasts

Brian D. Barnhart, Augustine Chuang, Jurandir J.Dalle Lucca, Steven Roberts, Frederick Liewehr, Anthony P. Joyce

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

The purpose of this study was to measure the cytotoxicity of six endodontic irrigants on cultured gingival fibroblasts using the CyQuant assay. Human gingival fibroblasts were grown in Dulbecco's Modified Eagle Medium (DMEM) containing 10% fetal bovine serum at 37°C and 5% CO2. At confluence, cells were split, plated in 96-well plates and incubated for 24-h to allow attachment. The following irrigants were tested at various concentrations: Sodium hypochlorite (NaOCl); iodine potassium-iodide (IKI); Betadine scrub (BS); calcium hydroxide [Ca(OH)2]; chlorine dioxide (SCD) and DMEM (positive control). Experimental groups were compared by the logarithmic difference between the clinical and LD50 concentrations of a particular irrigant. The results showed that IKI and Ca(OH)2 were significantly less cytotoxic than SCD, NaOCl, and BS. In conclusion, IKI and Ca(OH)2 are well tolerated by human gingival fibroblasts.

Original languageEnglish (US)
Pages (from-to)613-615
Number of pages3
JournalJournal of Endodontics
Volume31
Issue number8
DOIs
StatePublished - Jan 1 2005

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Endodontics
Povidone-Iodine
Eagles
Fibroblasts
Calcium Hydroxide
Sodium Hypochlorite
Lethal Dose 50
Serum
In Vitro Techniques

Keywords

  • Betadine scrub
  • Calcium hydroxide
  • Chlorine dioxide
  • Iodine potassium-iodide
  • Sodium hypochlorite

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

An in vitro evaluation of the cytotoxicity of various endodontic irrigants on human gingival fibroblasts. / Barnhart, Brian D.; Chuang, Augustine; Lucca, Jurandir J.Dalle; Roberts, Steven; Liewehr, Frederick; Joyce, Anthony P.

In: Journal of Endodontics, Vol. 31, No. 8, 01.01.2005, p. 613-615.

Research output: Contribution to journalArticle

Barnhart, Brian D. ; Chuang, Augustine ; Lucca, Jurandir J.Dalle ; Roberts, Steven ; Liewehr, Frederick ; Joyce, Anthony P. / An in vitro evaluation of the cytotoxicity of various endodontic irrigants on human gingival fibroblasts. In: Journal of Endodontics. 2005 ; Vol. 31, No. 8. pp. 613-615.
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