An In Vitro Evaluation of the Growth of Human Periodontal Ligament Fibroblasts after Exposure to a Methacrylate-based Endodontic Sealer

Erich P. Heitman, Anthony P. Joyce, James C. McPherson, Steven Roberts, Augustine Chuang

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

The cytotoxicity of Epiphany root canal sealer at various concentrations from 25-800 μg/mL on human periodontal ligament (HPDL) fibroblasts was evaluated at 1, 3, and 7 days. Controls included untreated cells and cells treated with the vehicle for Epiphany suspension. Fibroblast viability was assessed by 2 methods, crystal violet staining in 24-well plates and the fluorescence-based CyQUANT Cell Proliferation Assay in 96-well plates. Significant cytotoxicity against HPDL fibroblast growth by Epiphany was both time- and concentration-dependent. On day 1, 800 μg/mL, the highest concentration of Epiphany, showed significant cytotoxicity (P ≤ .001). By day 7, all concentrations greater than 25 μg/mL showed significant (P ≤ .05) loss of viability. This study demonstrated increased Epiphany cytotoxicity with an increase in concentration or exposure time.

Original languageEnglish (US)
Pages (from-to)186-189
Number of pages4
JournalJournal of Endodontics
Volume34
Issue number2
DOIs
StatePublished - Feb 1 2008

Fingerprint

Periodontal Ligament
Methacrylates
Endodontics
Fibroblasts
Growth
Gentian Violet
Dental Pulp Cavity
Suspensions
Fluorescence
Cell Proliferation
Staining and Labeling
In Vitro Techniques

Keywords

  • Cytotoxicity
  • Epiphany
  • root canal sealer

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

An In Vitro Evaluation of the Growth of Human Periodontal Ligament Fibroblasts after Exposure to a Methacrylate-based Endodontic Sealer. / Heitman, Erich P.; Joyce, Anthony P.; McPherson, James C.; Roberts, Steven; Chuang, Augustine.

In: Journal of Endodontics, Vol. 34, No. 2, 01.02.2008, p. 186-189.

Research output: Contribution to journalArticle

Heitman, Erich P. ; Joyce, Anthony P. ; McPherson, James C. ; Roberts, Steven ; Chuang, Augustine. / An In Vitro Evaluation of the Growth of Human Periodontal Ligament Fibroblasts after Exposure to a Methacrylate-based Endodontic Sealer. In: Journal of Endodontics. 2008 ; Vol. 34, No. 2. pp. 186-189.
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