An in vitro evaluation of the growth of human periodontal ligament fibroblasts after exposure to a 4-META-containing methacrylate-based endodontic sealer

Bradley Morrison, Stephanie Sidow, Kathleen McNally, James McPherson, Augustine Chuang

Research output: Contribution to journalArticle

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Abstract

Introduction: In this study we evaluated the cytotoxic effects of MetaSEAL, a 4-META-containing meth-acrylate-based endodontic sealer, on human periodontal ligament (HPDL) fibroblasts. There are a limited number of studies on the cytotoxic effects of MetaSEAL, and there are no studies on the cytotoxic effects of MetaSEAL on cells it might come into contact with in vivo. Methods: MetaSEAL concentrations of 25, 50, 100, 200, 400, and 800 μg/mL were exposed to HPDL fibroblast cultures and evaluated at 1, 3, 7, 14, and 21 days. Controls included untreated cells and cells treated with ethanol, the vehicle for MetaSEAL suspension. Crystal violet staining in 24-well plates and the fluorescence-based CyQUANT Cell Proliferation Assay in 96-well plates assessed fibroblast viability. Results: Significant cytotoxicity against HPDL growth by MetaSEAL was both time- and concentration-dependent. At day 1 there were no significant cytotoxic effects, whereas by day 3, 800 μg/mL concentration, by day 7, 200, 400, and 800 μg/mL concentrations, and by day 14, 50, 100, 200, 400, and 800 μg/mL concentrations were significantly cytotoxic. By day 21, all concentrations were significantly cytotoxic. These findings were confirmed by both the crystal violet and CyQUANT assays. Conclusions: MetaSEAL endodontic sealer has increasing HPDL cytotoxicity with both concentration and time exposure.

Original languageEnglish (US)
Pages (from-to)803-806
Number of pages4
JournalJournal of Endodontics
Volume37
Issue number6
DOIs
StatePublished - Jun 1 2011

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Periodontal Ligament
Methacrylates
Endodontics
Fibroblasts
Gentian Violet
Growth
Suspensions
Ethanol
Fluorescence
Cell Proliferation
Staining and Labeling
4-methacryloxyethyltrimellitic acid anhydride
In Vitro Techniques

Keywords

  • Human periodontal ligament fibroblasts
  • MetaSEAL
  • methacrylate resin-based sealer

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

An in vitro evaluation of the growth of human periodontal ligament fibroblasts after exposure to a 4-META-containing methacrylate-based endodontic sealer. / Morrison, Bradley; Sidow, Stephanie; McNally, Kathleen; McPherson, James; Chuang, Augustine.

In: Journal of Endodontics, Vol. 37, No. 6, 01.06.2011, p. 803-806.

Research output: Contribution to journalArticle

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AU - Chuang, Augustine

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