An incremental increase in the complexity of peptides bound to class II MHC changes the diversity of positively selected αβ TCRs

Rafal W Pacholczyk, P. Kraj, L. Ignatowicz

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10 Scopus citations

Abstract

Positive selection of the normal repertoire of TCRs results from low-avidity interactions with a set of self-peptides bound to the MHC molecules expressed by thymic epithelial cells. The contribution of the individual peptide to positive selection remains a matter of debate. Here, for the first time, we show that two covalent class II MHC-peptide complexes positively select different TCRs expressing a common transgenic TCRβ-chain and endogenous TCRα-chains. Simultaneous expression of both Ab-peptide complexes changed the diversity of positively selected TCRs, indicating an additive and possibly synergistic effect of various peptides in this process.

Original languageEnglish (US)
Pages (from-to)2357-2363
Number of pages7
JournalJournal of Immunology
Volume166
Issue number4
Publication statusPublished - Feb 15 2001

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ASJC Scopus subject areas

  • Immunology

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