An investigation of fibroblast mitochondria enzyme activity and respiration in response to metallic ions released from dental alloys

Regina L W Messer, J. E. Doeller, D. W. Kraus, L. C. Lucas

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Most cellular functions evaluated for biocompatibility are high-energy processes such as proliferation and therefore are not usually affected before a decrease in energy production is observed. Several studies have shown that metabolic functions are altered at much lower concentrations than several normally used biocompatibility tests such as viability. Therefore, the purpose of this study was to provide an in-depth evaluation of metallic ion effects on mitochondria function and thereby biocompatibility. These studies evaluated the mitochondrial function of human gingival fibroblasts exposed to the salt solutions of ions released from nickel-based dental alloys, particularly beryllium (Be2+), chromium (Cr6+ and Cr3+), nickel (Ni2+), and molybdenum (Mo6+). Mitochondrial function was examined by NADH:CoQ reductase activity, succinate dehydrogenase activity, and oxygen consumption. (C) 2000 John Wiley and Sons, Inc.

Original languageEnglish (US)
Pages (from-to)598-604
Number of pages7
JournalJournal of Biomedical Materials Research
Volume50
Issue number4
DOIs
StatePublished - May 9 2000

Fingerprint

Dental alloys
Dental Alloys
Mitochondria
Enzyme activity
Fibroblasts
Nickel
Respiration
Ions
Beryllium
Electron Transport Complex I
Biocompatibility
Molybdenum
Succinate Dehydrogenase
Chromium
Enzymes
Oxygen Consumption
Salts
Oxygen

Keywords

  • Biocompatibility, cell culture
  • Cytotoxicity, metabolic
  • Metal ions

ASJC Scopus subject areas

  • Biomaterials
  • Biomedical Engineering

Cite this

An investigation of fibroblast mitochondria enzyme activity and respiration in response to metallic ions released from dental alloys. / Messer, Regina L W; Doeller, J. E.; Kraus, D. W.; Lucas, L. C.

In: Journal of Biomedical Materials Research, Vol. 50, No. 4, 09.05.2000, p. 598-604.

Research output: Contribution to journalArticle

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