An RNAi-independent role of AP1-like stress response factor Pap1 in centromere and mating-type silencing in Schizosaccaromyces pombe

Ashok Kumar, Jagpreet Singh Nanda, Sharanjot Saini, Jagmohan Singh

Research output: Contribution to journalArticlepeer-review

Abstract

Gene silencing in S. pombe occurs by heterochromatin formation at the centromere (cen), mating-type (mat) and telomere loci. It is mediated by silencing factors including Swi6, Clr1-4, Rhp6 and Polα. RNAi pathway also plays a role in establishment of silencing at the mat and cen loci. Recently, the stress response factors, Atf1 and Pcr1were shown to play an RNAi-independent role in silencing at the mat3 locus through a cis-acting Atf1-binding site located within the repression element REIII and recruitment of the silencing factors Clr3 and Clr6. Another cis-acting site, named repression element REII abutting the mat2 locus, also establishes heterochromatin structure through Clr5 and histone deacetylases but independently of H3-Lys9-methylation and RNAi. Here, we report the occurrence of binding sites for another oxidative response factor, the pombe AP1-like factor Pap1, at the mating-type, centromere and telomere loci. By genetic studies we show that these sites play a role in silencing at the outer repeats of centromeres as well as mating-type locus and this effect is mediated through Pap1 binding site and interaction with and recruitment of the HP1/Swi6. Importantly, pap1Δ cells display a silencing defect even in absence of the oxidative stress. Such a role of Pap1 in heterochromatin formation may be evolutionarily conserved.

Original languageEnglish (US)
Article number74
JournalJournal of Biosciences
Volume46
Issue number3
DOIs
StatePublished - Sep 2021

Keywords

  • Fission yeast
  • heterochromatin
  • mating-type loci
  • oxidative stress
  • Pap1
  • silencing

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

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