Analysis of major histocompatibility complex class I gene transcription using oligonucleotide probes

Andrew L. Mellor

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Many highly homologous genes are present in the murine major histocompatibility complex (MHC) class I gene family. Consequently, it is difficult to distinguish between RNA transcripts of individual class I genes solely on the basis of nucleic acid hybridization analysis using DNA probes over 50 base pairs long. To avoid this problem, I have designed and synthesized a set of oligonucleotide probes capable of detecting transcripts of single class I genes in the MHC of C57BL/10 mice or sets of allelic class I genes at the same genetic locus in MHC disparate mouse strains. Using these probes, it is possible to determine the relative abundance of specific class I gene transcripts in a wide variety of cell and tissue types from inbred or MHC disparate mice. Examples of the use of these probes to detect different class I gene transcripts in cloned murine T cells, T cells transformed with Radiation Leukemia Virus, chemically induced thymoma cell lines and embryonic tissues are described. The results of these experiments are discussed in the light of possible roles of class I antigens in tumorigenesis or in early development.

Original languageEnglish (US)
Pages (from-to)207-220
Number of pages14
JournalMolecular and Cellular Probes
Volume1
Issue number3
DOIs
StatePublished - Jan 1 1987

Fingerprint

MHC Class I Genes
Oligonucleotide Probes
Major Histocompatibility Complex
Radiation Leukemia Virus
Nucleic Acid Hybridization
T-Lymphocytes
Histocompatibility Antigens Class I
Genetic Loci
Thymoma
DNA Probes
Inbred C57BL Mouse
Base Pairing
Carcinogenesis
RNA
Cell Line
Genes

Keywords

  • MHC genes
  • oligonucleotides

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology

Cite this

Analysis of major histocompatibility complex class I gene transcription using oligonucleotide probes. / Mellor, Andrew L.

In: Molecular and Cellular Probes, Vol. 1, No. 3, 01.01.1987, p. 207-220.

Research output: Contribution to journalArticle

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