Analysis of oncogenic activities of protein kinase D1 in head and neck squamous cell carcinoma

Liyong Zhang, Zhihong Li, Yehai Liu, Shuping Xu, Manuj Tandon, Brittany Appelboom, Courtney R. Lavalle, Simion I. Chiosea, Lin Wang, Malabika Sen, Vivian W.Y. Lui, Jennifer R. Grandis, Q. Jane Wang

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

Background: Head and neck squamous cell carcinoma (HNSCC) is the sixth leading cause of cancer death in the US. The protein kinase D (PKD) family has emerged as a promising target for cancer therapy with PKD1 being most intensively studied; however, its role in HNSCC has not been investigated. Methods: The expression of PKD was evaluated in human HNSCC by quantitative RT-PCR, Western blot and immunohistochemistry. Cell proliferation, wound healing, and matrigel invasion assays were performed upon siRNA-mediated knockdown of PKD1 in HNSCC cells, and subcutaneous xenograft mouse model was established by implantation of the stable doxycycline (Dox)-inducible PKD1 expression cell lines for analysis of tumorigenic activity in vivo. Results: PKD1 was frequently downregulated in HNSCC cell lines at both transcript and protein levels. In human HNSCC tissues, PKD1 was significantly down-regulated in localized tumors and metastases, and in patient-paired tumor tissues as compared to their normal counterparts, which was in part due to epigenetic modification of the PRKD1 gene. The function of PKD1 in HNSCC was analyzed using stable doxycycline-inducible cell lines that express native or constitutive-active PKD1. Upon induction, the rate of proliferation, survival, migration and invasion of HNSCC cells did not differ significantly between the control and PKD1 overexpressing cells in the basal state, and depletion of endogenous PKD1 did not impact the proliferation of HNSCC cells. However, the median growth rate of the subcutaneous HNSCC tumor xenografts over time was elevated with PKD1 induction, and the final tumor weight was significantly increased in Dox-induced vs. the non-induced tumors. Moreover, induced expression of PKD1 promoted bombesin-induced cell proliferation of HNSCC and resulted in sustained ERK1/2 activation in response to gastrin-releasing peptide or bombesin stimulation, suggesting that PKD1 potentiates GRP/bombesin-induced mitogenic response through the activation of ERK1/2 in HSNCC cells. Conclusions: Our study has identified PKD1 as a frequently downregulated gene in HNSCC, and functionally, under certain cellular context, may play a role in GRP/bombesin-induced oncogenesis in HNSCC.

Original languageEnglish (US)
Article number1107
JournalBMC Cancer
Volume18
Issue number1
DOIs
StatePublished - Nov 12 2018
Externally publishedYes

Keywords

  • GRP/bombesin
  • Head and neck cancer
  • PKD1
  • Proliferation
  • Tumor growth

ASJC Scopus subject areas

  • Oncology
  • Genetics
  • Cancer Research

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