Analysis of Rab1 Function in Cardiomyocyte Growth

Catalin M. Filipeanu, Fuguo Zhou, Guangyu Wu

Research output: Chapter in Book/Report/Conference proceedingChapter

8 Citations (Scopus)

Abstract

Protein transport between intracellular organelles is coordinated by Rab GTPases. As an initial approach to defining the function of Rab GTPases in cardiomyocytes, our laboratory focused on Rab1, which regulates protein transport specifically from the endoplasmic reticulum (ER) to the Golgi apparatus. Our studies have demonstrated that adenovirus-driven expression of Rab1 promotes cell growth of primary cultures of neonatal cardiomyocytes in vitro and that transgenic expression of Rab1 in the myocardium induces cardiac hypertrophy in mouse hearts in vivo. These data provide strong evidence implicating that ER-to-Golgi protein transport functions as a regulatory site for control of cardiomyocyte growth. Here we describe a sets of methods used in our laboratory to characterize the function of Rab1 GTPase in modulating cardiac myocyte growth.

Original languageEnglish (US)
Title of host publicationSmall GTPases in Disease, Part A
EditorsWilliam Balch, Alan Hall, Channing Der
Pages217-226
Number of pages10
DOIs
StatePublished - Sep 24 2008

Publication series

NameMethods in Enzymology
Volume438
ISSN (Print)0076-6879

Fingerprint

rab GTP-Binding Proteins
Cardiac Myocytes
Protein Transport
Growth
Endoplasmic Reticulum
Proteins
GTP Phosphohydrolases
Cell growth
Cell culture
Primary Cell Culture
Cardiomegaly
Golgi Apparatus
Adenoviridae
Organelles
Myocardium

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

Cite this

Filipeanu, C. M., Zhou, F., & Wu, G. (2008). Analysis of Rab1 Function in Cardiomyocyte Growth. In W. Balch, A. Hall, & C. Der (Eds.), Small GTPases in Disease, Part A (pp. 217-226). (Methods in Enzymology; Vol. 438). https://doi.org/10.1016/S0076-6879(07)38015-4

Analysis of Rab1 Function in Cardiomyocyte Growth. / Filipeanu, Catalin M.; Zhou, Fuguo; Wu, Guangyu.

Small GTPases in Disease, Part A. ed. / William Balch; Alan Hall; Channing Der. 2008. p. 217-226 (Methods in Enzymology; Vol. 438).

Research output: Chapter in Book/Report/Conference proceedingChapter

Filipeanu, CM, Zhou, F & Wu, G 2008, Analysis of Rab1 Function in Cardiomyocyte Growth. in W Balch, A Hall & C Der (eds), Small GTPases in Disease, Part A. Methods in Enzymology, vol. 438, pp. 217-226. https://doi.org/10.1016/S0076-6879(07)38015-4
Filipeanu CM, Zhou F, Wu G. Analysis of Rab1 Function in Cardiomyocyte Growth. In Balch W, Hall A, Der C, editors, Small GTPases in Disease, Part A. 2008. p. 217-226. (Methods in Enzymology). https://doi.org/10.1016/S0076-6879(07)38015-4
Filipeanu, Catalin M. ; Zhou, Fuguo ; Wu, Guangyu. / Analysis of Rab1 Function in Cardiomyocyte Growth. Small GTPases in Disease, Part A. editor / William Balch ; Alan Hall ; Channing Der. 2008. pp. 217-226 (Methods in Enzymology).
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