Analysis of Rab1 Function in Cardiomyocyte Growth

Catalin M. Filipeanu, Fuguo Zhou, Guangyu Wu

Research output: Chapter in Book/Report/Conference proceedingChapter

9 Scopus citations

Abstract

Protein transport between intracellular organelles is coordinated by Rab GTPases. As an initial approach to defining the function of Rab GTPases in cardiomyocytes, our laboratory focused on Rab1, which regulates protein transport specifically from the endoplasmic reticulum (ER) to the Golgi apparatus. Our studies have demonstrated that adenovirus-driven expression of Rab1 promotes cell growth of primary cultures of neonatal cardiomyocytes in vitro and that transgenic expression of Rab1 in the myocardium induces cardiac hypertrophy in mouse hearts in vivo. These data provide strong evidence implicating that ER-to-Golgi protein transport functions as a regulatory site for control of cardiomyocyte growth. Here we describe a sets of methods used in our laboratory to characterize the function of Rab1 GTPase in modulating cardiac myocyte growth.

Original languageEnglish (US)
Title of host publicationSmall GTPases in Disease, Part A
EditorsWilliam Balch, Alan Hall, Channing Der
Pages217-226
Number of pages10
DOIs
StatePublished - Sep 24 2008
Externally publishedYes

Publication series

NameMethods in Enzymology
Volume438
ISSN (Print)0076-6879

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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    Filipeanu, C. M., Zhou, F., & Wu, G. (2008). Analysis of Rab1 Function in Cardiomyocyte Growth. In W. Balch, A. Hall, & C. Der (Eds.), Small GTPases in Disease, Part A (pp. 217-226). (Methods in Enzymology; Vol. 438). https://doi.org/10.1016/S0076-6879(07)38015-4