Analysis of rat calvaria defects implanted with a platelet-rich plasma preparation: Radiographic observations

Mary E. Pryor, Jie Yang, Giuseppe Polimeni, Ki Tae Koo, Michael J. Hartman, Howard Gross, Alexis Agelan, Joanne M. Manns, Ulf M E Wikesjö

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Background: Platelet-rich plasma (PRP) harbors growth factors identified in bone. It has been suggested that these factors enhance osteogenesis. The objective of this study was to conduct a radiographic evaluation on local bone formation following surgical implantation of a PRP preparation using a critical-size rat calvaria defect model. Methods: Thirty 22-week-old male Sprague-Dawley rats were used. The PRP preparation was obtained from 10 ml of whole blood drawn from one age-matched donor rat. The preparation was processed by gradient density centrifugation and stored at -80°C until use. Using aseptic techniques, the PRP preparation soak-loaded onto an absorbable collagen sponge (ACS) carrier or ACS alone was surgically implanted into contralateral critical-size 6 mm rat calvaria osteotomies in 18 animals. Twelve animals received ACS alone versus sham surgery in contralateral defects. Animals were sacrificed at 4 and 8 weeks when biopsies were collected and radiographs were obtained using a standardized protocol. Three masked examiners independently evaluated the radiographic images of the defect sites. Examiner reproducibility was examined by repeat evaluation of all defect sites (r = 0.6; P<0.0001). Results: The animals were maintained without adverse events. Defect sites in two animals receiving ACS versus sham surgery (4-week healing interval) were not evaluated due to specimen damage. Seventy-five percent of the sites (PRP/ACS or ACS) exhibited partial closure at 4 weeks; one site (ACS) exhibited full closure without significant differences between protocols (P = 0.1797). Fifty percent of the sites receiving PRP/ACS exhibited full closure and 20% partial closure at 8 weeks versus 20% and 80%, respectively, for the ACS control (P= 0.7532). There were no noteworthy differences between sites receiving ACS versus sham surgery at 4 or 8 weeks. Conclusion: The results suggest that the PRP preparation does not have a significant effect on osteogenesis.

Original languageEnglish (US)
Pages (from-to)1287-1292
Number of pages6
JournalJournal of periodontology
Volume76
Issue number8
DOIs
StatePublished - Aug 1 2005

Fingerprint

Platelet-Rich Plasma
Porifera
Skull
Collagen
Osteogenesis
Density Gradient Centrifugation
Osteotomy
Sprague Dawley Rats
Intercellular Signaling Peptides and Proteins
Biopsy
Bone and Bones

Keywords

  • Animal studies
  • Bone and bones
  • Growth factors
  • Osteogenesis
  • Plasma, platelet-rich
  • Tissue engineering

ASJC Scopus subject areas

  • Periodontics

Cite this

Pryor, M. E., Yang, J., Polimeni, G., Koo, K. T., Hartman, M. J., Gross, H., ... Wikesjö, U. M. E. (2005). Analysis of rat calvaria defects implanted with a platelet-rich plasma preparation: Radiographic observations. Journal of periodontology, 76(8), 1287-1292. https://doi.org/10.1902/jop.2005.76.8.1287

Analysis of rat calvaria defects implanted with a platelet-rich plasma preparation : Radiographic observations. / Pryor, Mary E.; Yang, Jie; Polimeni, Giuseppe; Koo, Ki Tae; Hartman, Michael J.; Gross, Howard; Agelan, Alexis; Manns, Joanne M.; Wikesjö, Ulf M E.

In: Journal of periodontology, Vol. 76, No. 8, 01.08.2005, p. 1287-1292.

Research output: Contribution to journalArticle

Pryor, ME, Yang, J, Polimeni, G, Koo, KT, Hartman, MJ, Gross, H, Agelan, A, Manns, JM & Wikesjö, UME 2005, 'Analysis of rat calvaria defects implanted with a platelet-rich plasma preparation: Radiographic observations', Journal of periodontology, vol. 76, no. 8, pp. 1287-1292. https://doi.org/10.1902/jop.2005.76.8.1287
Pryor, Mary E. ; Yang, Jie ; Polimeni, Giuseppe ; Koo, Ki Tae ; Hartman, Michael J. ; Gross, Howard ; Agelan, Alexis ; Manns, Joanne M. ; Wikesjö, Ulf M E. / Analysis of rat calvaria defects implanted with a platelet-rich plasma preparation : Radiographic observations. In: Journal of periodontology. 2005 ; Vol. 76, No. 8. pp. 1287-1292.
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abstract = "Background: Platelet-rich plasma (PRP) harbors growth factors identified in bone. It has been suggested that these factors enhance osteogenesis. The objective of this study was to conduct a radiographic evaluation on local bone formation following surgical implantation of a PRP preparation using a critical-size rat calvaria defect model. Methods: Thirty 22-week-old male Sprague-Dawley rats were used. The PRP preparation was obtained from 10 ml of whole blood drawn from one age-matched donor rat. The preparation was processed by gradient density centrifugation and stored at -80°C until use. Using aseptic techniques, the PRP preparation soak-loaded onto an absorbable collagen sponge (ACS) carrier or ACS alone was surgically implanted into contralateral critical-size 6 mm rat calvaria osteotomies in 18 animals. Twelve animals received ACS alone versus sham surgery in contralateral defects. Animals were sacrificed at 4 and 8 weeks when biopsies were collected and radiographs were obtained using a standardized protocol. Three masked examiners independently evaluated the radiographic images of the defect sites. Examiner reproducibility was examined by repeat evaluation of all defect sites (r = 0.6; P<0.0001). Results: The animals were maintained without adverse events. Defect sites in two animals receiving ACS versus sham surgery (4-week healing interval) were not evaluated due to specimen damage. Seventy-five percent of the sites (PRP/ACS or ACS) exhibited partial closure at 4 weeks; one site (ACS) exhibited full closure without significant differences between protocols (P = 0.1797). Fifty percent of the sites receiving PRP/ACS exhibited full closure and 20{\%} partial closure at 8 weeks versus 20{\%} and 80{\%}, respectively, for the ACS control (P= 0.7532). There were no noteworthy differences between sites receiving ACS versus sham surgery at 4 or 8 weeks. Conclusion: The results suggest that the PRP preparation does not have a significant effect on osteogenesis.",
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AU - Yang, Jie

AU - Polimeni, Giuseppe

AU - Koo, Ki Tae

AU - Hartman, Michael J.

AU - Gross, Howard

AU - Agelan, Alexis

AU - Manns, Joanne M.

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KW - Plasma, platelet-rich

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