Analysis of wilms tumors using SNP mapping array-based comparative genomic hybridization

Research output: Contribution to journalArticle

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Abstract

Wilms tumor (WT) has been a model to study kidney embryogenesis and tumorigenesis and, although associated with hereditary, cancer predisposition syndromes, the majority of tumors occur sporadically. To analyze genetic changes in WT we have defined copy number changes and loss of heterozygosity in 56 Wilms tumors using high resolution oligonucleotide arrays at a average resolution of ~12 Kb. Consistent deletions were seen on chromosomes 1p, 4q, 7p, 9q, 11p, 11q, 14q, 16q, and 21q. High frequency gains were seen for 1q and lower frequency gains were seen on 7q and chromosomes 8, 12 and 18. The high resolution provided by the SNP mapping arrays has defined minimal regions of deletion for many of these LOH events. Analysis of CNAs by tumor stage show relatively stable karyotypes in stage 1 tumors and more complex aCGH profiles in tumors from stages 3-5.

Original languageEnglish (US)
Article numbere18941
JournalPloS one
Volume6
Issue number4
DOIs
StatePublished - May 4 2011

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comparative genomic hybridization
Comparative Genomic Hybridization
Wilms Tumor
Single Nucleotide Polymorphism
Tumors
neoplasms
Neoplasms
Hereditary Neoplastic Syndromes
Chromosomes, Human, Pair 18
Chromosomes, Human, Pair 12
Chromosomes, Human, Pair 8
Chromosomes
Loss of Heterozygosity
Oligonucleotide Array Sequence Analysis
Karyotype
Embryonic Development
Carcinogenesis
chromosomes
Kidney
Oligonucleotides

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • General

Cite this

Analysis of wilms tumors using SNP mapping array-based comparative genomic hybridization. / Hawthorn, Lesleyann; Cowell, John K.

In: PloS one, Vol. 6, No. 4, e18941, 04.05.2011.

Research output: Contribution to journalArticle

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